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Literature summary for 3.4.24.67 extracted from
- Purification and characterization of hatching enzyme from flounder Paralichthys olivaceus (2006), Fish Physiol. Biochem., 32, 35-42.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bestatin | - |
Paralichthys olivaceus |  |
| chymostatin | - |
Paralichthys olivaceus | |
| Cu2+ | about 95% inhibition at 5 mM | Paralichthys olivaceus | |
| EDTA | 90% inhibition at 20 mM, 75% at 10 mM | Paralichthys olivaceus | |
| iodoacetamide | - |
Paralichthys olivaceus | |
| leupeptin | - |
Paralichthys olivaceus | |
| lima bean trypsin-chymotrypsin inhibitor | LBTI | Paralichthys olivaceus | |
| additional information | the enzyme is very sensitive to trypsin-specific inhibitors, especially serine protease-specific inhibitors | Paralichthys olivaceus | |
| NEM | - |
Paralichthys olivaceus | |
| ovomucoid | - |
Paralichthys olivaceus | |
| p-APMSF | - |
Paralichthys olivaceus | |
| pepstatin A | - |
Paralichthys olivaceus | |
| PMSF | - |
Paralichthys olivaceus | |
| soybean trypsin-chymotrypsin inhibitor | SBTI | Paralichthys olivaceus | |
| TLCK | - |
Paralichthys olivaceus | |
| TPCK | - |
Paralichthys olivaceus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.28 | - |
casein | pH 7.0, 35°C | Paralichthys olivaceus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 450% activation at 2 mM | Paralichthys olivaceus | |
| Mg2+ | 180% activation at 2 mM | Paralichthys olivaceus | |
| additional information | no effect by Zn2+ at 5 mM | Paralichthys olivaceus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34800 | - |
x * 34800, SDS-PAGE | Paralichthys olivaceus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chorion + H2O | Paralichthys olivaceus | specific substrate, the hatching enzyme is secreted from embryos for digesting their protective extracellular coats | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paralichthys olivaceus | - |
flounder | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 40fold from embryos by gel filtration and anion exchange chromatography | Paralichthys olivaceus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | secretion of the enzyme | Paralichthys olivaceus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
purified native enzyme, 295.88 A280/mg/min | Paralichthys olivaceus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Paralichthys olivaceus | ? | - |
? | |
| chorion + H2O | specific substrate, the hatching enzyme is secreted from embryos for digesting their protective extracellular coats | Paralichthys olivaceus | ? | - |
? | |
| chorion + H2O | of Paralichthys olivaceus, specific substrate | Paralichthys olivaceus | ? | - |
? | |
| additional information | the enzyme is a trypsin-type serine protease | Paralichthys olivaceus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34800, SDS-PAGE | Paralichthys olivaceus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hatching enzyme | - |
Paralichthys olivaceus |
| PHE | - |
Paralichthys olivaceus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Paralichthys olivaceus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
temperature profile | Paralichthys olivaceus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Paralichthys olivaceus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH profile | Paralichthys olivaceus |
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