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Literature summary for 3.4.24.64 extracted from

  • Marcondes, M.F.; Alves, F.M.; Assis, D.M.; Hirata, I.Y.; Juliano, L.; Oliveira, V.; Juliano, M.A.
    Substrate specificity of mitochondrial intermediate peptidase analysed by a support-bound peptide library (2015), FEBS open bio, 5, 429-436 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.64

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00085
-
 Abz-TTKLKAAK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.000941
-
 Abz-FRSGQPLQNKVQLQ-ED(Dnp) pH 8.0, 25°C Homo sapiens
0.0011
-
 Abz-IKQSSLLK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.0013
-
 Abz-NLMKKSTK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.0013
-
 Abz-FSSKTTVK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.0018
-
 Abz-VRNFRSGQPLQNKVQ-ED(Dnp) pH 8.0, 25°C Homo sapiens
0.0019
-
 Abz-VISSRLEK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.0029
-
 Abz-QPLQNKVQ-ED(Dnp) pH 8.0, 25°C Homo sapiens
0.0032
-
 Abz-QPLQAKVQ-ED(Dnp) pH 8.0, 25°C Homo sapiens
0.0034
-
 Abz-FQTKVAAK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.0042
-
 Abz-MTAALKTK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.0051
-
 Abz-QPLANKVQ-ED(Dnp) pH 8.0, 25°C Homo sapiens
0.0062
-
 Abz-WTTGGKAK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.01
-
 Abz-VAAQTKTK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q10713 AND O75439 Q10713: mitochondrial-processing peptidase subunit alpha, O75439: mitochondrial-processing peptidase subunit beta
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-FQTKVAAK(Dnp)-NH2 + H2O
-
 Homo sapiens Abz-FQ + TKVAAK(Dnp)-NH2
-
 ?
Abz-FRSGQPLQNKVQLQ-ED(Dnp) + H2O
-
 Homo sapiens Abz-FRSGQPLQ + NKVQLQ-ED(Dnp)
-
 ?
Abz-FSSKTTVK(Dnp)-NH2 + H2O
-
 Homo sapiens Abz-FS + SKTTVK(Dnp)-NH2
-
 ?
Abz-IKQSSLLK(Dnp)-NH2 + H2O
-
 Homo sapiens Abz-IKQ + SSLLK(Dnp)-NH2
-
 ?
Abz-MTAALKTK(Dnp)-NH2 + H2O cleavage sites: 2-aminobenzoyl-MT-/-AALK-/-TK-(N-(2,4-dinitrophenyl)-ethylenediamine)-NH2 Homo sapiens Abz-MT + AALK + TK(Dnp)-NH2
-
 ?
Abz-NLMKKSTK(Dnp)-NH2 + H2O
-
 Homo sapiens Abz-NLM + KKSTK(Dnp)-NH2
-
 ?
Abz-QPLANKVQ-ED(Dnp) + H2O
-
 Homo sapiens Abz-QPLA + NKVQ-ED(Dnp)
-
 ?
Abz-QPLQAKVQ-ED(Dnp) + H2O
-
 Homo sapiens Abz-QPLQ + AKVQ-ED(Dnp)
-
 ?
Abz-QPLQNKVQ-ED(Dnp) + H2O
-
 Homo sapiens Abz-QPLQ + NKVQ-ED(Dnp)
-
 ?
Abz-TTKLKAAK(Dnp)-NH2 + H2O cleavage sites: 2-aminobenzoyl-TTKL-/-K-/-A-/-AK-(N-(2,4-dinitrophenyl)-ethylenediamine)-NH2 Homo sapiens Abz-TTKL + L-Lys + L-Ala + AK(Dnp)-NH2
-
 ?
Abz-VAAQTKTK(Dnp)-NH2 + H2O
-
 Homo sapiens Abz-VAA + QTKTK(Dnp)-NH2
-
 ?
Abz-VISSRLEK(Dnp)-NH2 + H2O
-
 Homo sapiens Abz-VIS + SRLEK(Dnp)-NH2
-
 ?
Abz-VRNFRSGQPLQNKVQ-ED(Dnp) + H2O
-
 Homo sapiens Abz-VRNFRSGQPLQ + NKVQ-ED(Dnp)
-
 ?
Abz-WTTGGKAK(Dnp)-NH2 + H2O
-
 Homo sapiens Abz-WT + TGGKAK(Dnp)-NH2
-
 ?
mitochondrial matrix proteins + H2O
-
 Homo sapiens ?
-
 ?
additional information the enzyme shows a preference for uncharged polar residues at positions P1 and P1 and nonpolar residues at the substrate P3, P2, P2' and P3' positions Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
MPP
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0033
-
 Abz-WTTGGKAK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.028
-
 Abz-NLMKKSTK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.036
-
 Abz-FSSKTTVK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.05
-
 Abz-VAAQTKTK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.055
-
 Abz-IKQSSLLK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.057
-
 Abz-FQTKVAAK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.057
-
 Abz-QPLANKVQ-ED(Dnp) pH 8.0, 25°C Homo sapiens
0.061
-
 Abz-QPLQNKVQ-ED(Dnp) pH 8.0, 25°C Homo sapiens
0.07
-
 Abz-TTKLKAAK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.083
-
 Abz-VISSRLEK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.0943
-
 Abz-QPLQAKVQ-ED(Dnp) pH 8.0, 25°C Homo sapiens
0.122
-
 Abz-FRSGQPLQNKVQLQ-ED(Dnp) pH 8.0, 25°C Homo sapiens
0.19
-
 Abz-MTAALKTK(Dnp)-NH2 pH 8.0, 25°C Homo sapiens
0.223
-
 Abz-VRNFRSGQPLQNKVQ-ED(Dnp) pH 8.0, 25°C Homo sapiens

General Information

General Information Comment Organism
physiological function most important processing enzyme that acts on proteins directed to the inner membrane intermembrane space or mitochondrial matrix. Most of the proteins targeted to the mitochondrial matrix or inner membrane present a single cleavage by mitochondrial processing peptidase Homo sapiens