Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme and enzyme mutants in DELTA atp23cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E168Q | site-directed mutation in the consensus metal-binding site, the active site residue mutant is proteolytically inactive, but shows processing of the Atp6 subunit of pre-F1FO-ATP synthase | Saccharomyces cerevisiae |
H167A | site-directed mutation in the consensus metal-binding site, the mutant does not show processing of the Atp6 subunit of pre-F1FO-ATP synthase | Saccharomyces cerevisiae |
H171A | site-directed mutation in the consensus metal-binding site, the mutant does not show processing of the Atp6 subunit of pre-F1FO-ATP synthase | Saccharomyces cerevisiae |
additional information | deletion of ATP23 completely inhibits the assembly of the mitochondrial-encoded FO-subunits into the F1FO-ATP synthase, which is observed in wild-type mitochondria | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | intermembrane space | Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | consensus metal-binding motif HEliH, formed by amino acid residues 167-171 of Atp23 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | the enzyme genetically interacts with prohibitins in the inner mitochondrial membrane and links the function of prohibitins to the F1FO-ATP synthase complex | ? | - |
? | |
pre-F1FO-ATP synthase + H2O | Saccharomyces cerevisiae | on one hand, Atp23 serves as a processing peptidase and mediates the maturation of the mitochondrially-encoded FO-subunit Atp6 after its insertion into the inner membrane, on the other hand, independent of its proteolytic activity, Atp23 promotes the association of mature Atp6 with Atp9 oligomers with chaperone activity, overview, the assembly step is thus under the control of two substrate-specific chaperones, Atp10 and Atp23, which act on opposite sides of the inner membrane, modelling of assembly, overview | mature F1FO-ATP synthase + prepeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
wild-type strain S288c and derivatives | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme genetically interacts with prohibitins in the inner mitochondrial membrane and links the function of prohibitins to the F1FO-ATP synthase complex | Saccharomyces cerevisiae | ? | - |
? | |
pre-F1FO-ATP synthase + H2O | on one hand, Atp23 serves as a processing peptidase and mediates the maturation of the mitochondrially-encoded FO-subunit Atp6 after its insertion into the inner membrane, on the other hand, independent of its proteolytic activity, Atp23 promotes the association of mature Atp6 with Atp9 oligomers with chaperone activity, overview, the assembly step is thus under the control of two substrate-specific chaperones, Atp10 and Atp23, which act on opposite sides of the inner membrane, modelling of assembly, overview | Saccharomyces cerevisiae | mature F1FO-ATP synthase + prepeptide | - |
? | |
pre-F1FO-ATP synthase + H2O | putative catalytically active Glu168 | Saccharomyces cerevisiae | mature F1FO-ATP synthase + prepeptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Atp23 | - |
Saccharomyces cerevisiae |
processing peptidase | - |
Saccharomyces cerevisiae |