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Literature summary for 3.4.24.64 extracted from

  • Kitada, S.; Kojima, K.; Shimokata, K.; Ogishima, T.; Ito, A.
    Glutamate residues required for substrate binding and cleavage activity in mitochondrial processing peptidase (1998), J. Biol. Chem., 273, 32547-32553.
    View publication on PubMed
Search for more literature for 3.4.24.64

Protein Variants

Protein Variants Comment Organism
E129A site-directed mutagenesis Rattus norvegicus
E136A site-directed mutagenesis Rattus norvegicus
E136D site-directed mutagenesis Rattus norvegicus
E139A site-directed mutagenesis Rattus norvegicus
E47A site-directed mutagenesis Rattus norvegicus
E47D site-directed mutagenesis Rattus norvegicus
E59Q site-directed mutagenesis Rattus norvegicus
E75A site-directed mutagenesis Rattus norvegicus
E75D site-directed mutagenesis Rattus norvegicus
E77A site-directed mutagenesis Rattus norvegicus
E77D site-directed mutagenesis Rattus norvegicus
E79A site-directed mutagenesis Rattus norvegicus
E79D site-directed mutagenesis Rattus norvegicus
H56R site-directed mutagenesis Rattus norvegicus
H60R site-directed mutagenesis Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000063
-
 MDH1-21 pH 7.5, 25°C, mutant E47D Rattus norvegicus
0.000074
-
 MDH1-21 pH 7.5, 25°C, wild type Rattus norvegicus
0.000085
-
 MDH1-21 pH 7.5, 25°C, mutant E129A Rattus norvegicus
0.00011
-
 MDH1-21 pH 7.5, 25°C, mutant E139Aand E75D Rattus norvegicus
0.00013
-
 MDH1-21 pH 7.5, 25°C, mutant E129D Rattus norvegicus
0.00021
-
 MDH1-21 pH 7.5, 25°C, mutant E77D Rattus norvegicus
0.00023
-
 MDH1-21 pH 7.5, 25°C, mutant E75A Rattus norvegicus
0.00024
-
 MDH1-21 pH 7.5, 25°C, mutant E77A Rattus norvegicus
0.00029
-
 MDH1-21 pH 7.5, 25°C, mutant E47A Rattus norvegicus
0.00045
-
 MDH1-21 pH 7.5, 25°C, mutant E79D Rattus norvegicus
0.00091
-
 MDH1-21 pH 7.5, 25°C, mutant E79A Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 rat
-
Saccharomyces cerevisiae
-
 yeast
-

Purification (Commentary)

Purification (Comment) Organism
histidine-tagged alpha-MPP subunit Saccharomyces cerevisiae
histidine-tagged MPP subunits Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amino benzoyl-LARPVGAALRRSFSTY(NO2)AQNN + H2O
-
 Rattus norvegicus ?
-
 ?
amino benzoyl-LARPVGAALRRSFSTY(NO2)AQNN + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
C-DACMDH + H2O
-
 Rattus norvegicus ?
-
 ?
MDH1-21 + H2O synthetic peptide substrate Rattus norvegicus ?
-
 ?
N-DACMDH + H2O
-
 Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
MPP
-
 Rattus norvegicus
MPP
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0016
-
 MDH1-21 pH 7.5, 25°C, mutant E79A Rattus norvegicus
0.016
-
 MDH1-21 pH 7.5, 25°C, mutant E79D Rattus norvegicus
0.06
-
 MDH1-21 pH 7.5, 25°C, mutant E129A Rattus norvegicus
0.163
-
 MDH1-21 pH 7.5, 25°C, mutant E129D Rattus norvegicus
0.3
-
 MDH1-21 pH 7.5, 25°C, mutant E47A Rattus norvegicus
0.533
-
 MDH1-21 pH 7.5, 25°C, mutant E77D Rattus norvegicus
0.7
-
 MDH1-21 pH 7.5, 25°C, mutant E47D Rattus norvegicus
0.833
-
 MDH1-21 pH 7.5, 25°C, mutant E75D Rattus norvegicus
0.916
-
 MDH1-21 pH 7.5, 25°C, mutant E77A Rattus norvegicus
1.33
-
 MDH1-21 pH 7.5, 25°C, mutant E139A Rattus norvegicus
1.4
-
 MDH1-21 pH 7.5, 25°C, wild type Rattus norvegicus
6.08
-
 MDH1-21 pH 7.5, 25°C, mutant E139A Rattus norvegicus
6.08
-
 MDH1-21 pH 7.5, 25°C, mutant E75A Rattus norvegicus
6.08
-
 MDH1-21 pH 7.5, 25°C, mutant E75D Rattus norvegicus
6.08
-
 MDH1-21 pH 7.5, 25°C, mutant E77A Rattus norvegicus
6.08
-
 MDH1-21 pH 7.5, 25°C, mutant E77D Rattus norvegicus