Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Neurospora crassa |
Neurospora crassa (alpha-MPP) | Neurospora crassa |
General Stability | Organism |
---|---|
Stable to 0.3-0.7 M urea | Neurospora crassa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | pb2(1-20) | Neurospora crassa | |
Peptide pb2(15-34) | derived from prepeptide of cytochrome b2 peptide pb2(1-31) | Neurospora crassa | |
Prepeptide pb2(1-31) | derived from cytochrome b2 presequence; kinetics | Neurospora crassa | |
Prepeptide pc1(1-36) | derived from cytochrome c1 presequence | Neurospora crassa | |
Prepeptide pF1beta(1-32) | derived from beta-subunit of F1-ATPase presequence | Neurospora crassa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | beta-MPP, identical with core I protein of ubiquinol cytochrome c reductase complex | Neurospora crassa | 16020 | - |
mitochondrion | beta-MPP: membrane-associated | Neurospora crassa | 5739 | - |
soluble | - |
Neurospora crassa | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant alpha-MPP and native beta-MPP | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chimeric preproteins derived from precursor of cytochrome b2 fused to dehydrofolate reductase + H2O | e.g. pb2delta19(167)-DHFR, cleavage site: Arg30-Xaa31-+-Xaa32, the term -+- depicts the point of cleavage | Neurospora crassa | Pb2(1-31) + ib2delta19(167)-DHFR processed protein | no further cleavage | ? | |
additional information | processing activity is optimal at a 1:1 molar ratio of alpha- and beta-MPP | Neurospora crassa | ? | - |
? | |
Precytochrome b2-mouse dehydrofolate reductase fusion protein + H2O | i.e. artificial fusion protein containing 22 amino acid presequence of cytochrome oxidase subunit IV fused to mouse dehydrofolate reductase, substitution of Arg2 or Tyr1 of matrix targeting sequence of cytochrome b2 prevents processing | Neurospora crassa | 31-aminoacid presequence + cytochrome b2-mouse dehydrofolate reductase protein | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | processing activity is optimal at a 1:1 molar ratio of alpha- and beta-MPP | Neurospora crassa |
More | subunits do not form a stable complex | Neurospora crassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
- |
Neurospora crassa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 8.5 | about half-maximal activity at pH 6.3 and about 90% of maximal activity at pH 8.5 | Neurospora crassa |