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Literature summary for 3.4.24.63 extracted from

  • Jaeckle, F.; Schmidt, F.; Wichert, R.; Arnold, P.; Prox, J.; Mangold, M.; Ohler, A.; Pietrzik, C.U.; Koudelka, T.; Tholey, A.; Guetschow, M.; Stirnberg, M.; Becker-Pauly, C.
    Metalloprotease meprin beta is activated by transmembrane serine protease matriptase-2 at the cell surface thereby enhancing APP shedding (2015), Biochem. J., 470, 91-103 .
    View publication on PubMed
Search for more literature for 3.4.24.63

Activating Compound

Activating Compound Comment Organism Structure
additional information meprin beta is expressed as an inactive zymogen and requires proteolytic maturation by tryptic serine proteases, e.g. by matriptase-2 (MT2), but not by TMPRSS4 or pancreatic trypsin Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene Mep1B, recombinant expression of N-terminally His-tagged meprin beta in HEK-293 cells, recombinant expression of meprin beta in COS-7 cells and coexpression with matrilysin-2 or pancreatic trypsin Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
fetuin-A
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Homo sapiens 9986
-
membrane
-
 Homo sapiens 16020
-
additional information meprin beta is a type 1 transmembrane protein, but can be released from the cell surface by ectodomain shedding Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a zinc metalloprotease Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amyloid precursor protein + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16820
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein deglycosylation of recombinant meprin beta by PNGaseF Homo sapiens
proteolytic modification metalloprotease meprin beta is activated by transmembrane serine protease matriptase-2 (MT2) at the cell surface. No processing by TMPRSS4 or pancreatic trypsin. The cleavage site for maturation within the propeptide of meprin beta is in close proximity to the plasma membrane, crystal structure analysis, PDB ID 4GWM. Proteomic identification of the MT2-dependent cleavage in pro-meprin beta by mass spectrometry Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged meprin beta from HEK-293 cells by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methyloxycoumarin-4-yl)acetyl-EDEDED-(K-epsilon-2,4-dinitrophenyl) + H2O
-
 Homo sapiens ?
-
 ?
amyloid precursor protein + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
cell surface sheddase
-
 Homo sapiens
meprin beta
-
 Homo sapiens

General Information

General Information Comment Organism
metabolism metalloprotease meprin beta is activated by transmembrane serine protease matriptase-2 at the cell surface thereby enhancing amyloid precursor protein (APP) shedding Homo sapiens
additional information molecular structure modelling of promeprin beta nd meprin beta, overview Homo sapiens
physiological function protease meprin beta is expressed as an inactive zymogen and requires proteolytic maturation by tryptic serine proteases. Maturation of full-length meprin beta is required for its activity as a cell surface sheddase, releasing the ectodomains of transmembrane proteins, e.g. amyloid precursor protein (APP) Homo sapiens