Literature summary for 3.4.24.63 extracted from

Schulze, A.; Wermann, M.; Demuth, H.U.; Yoshimoto, T.; Ramsbeck, D.; Schlenzig, D.; Schilling, S.
Continuous assays for meprin alpha and beta using prolyl tripeptidyl aminopeptidase (PtP) from Porphyromonas gingivalis (2018), Anal. Biochem., 559, 11-16 .

Search for more literature for 3.4.24.63

Cloned(Commentary)

Cloned (Comment)	Organism
gene MEP1B, recombinant expression of meprin beta in Pichia pastoris strain X-33	Homo sapiens
gene MEP1B, recombinant expression of N-terminally His6-tagged meprin beta Pichia pastoris strain X33	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
3-[[(3-carboxyphenyl)sulfonyl-[2-(hydroxyamino)-2-oxo-ethyl]amino]methyl]benzoic acid	hyperbolic noncompetitive/mixed-type inhibition; mixed type inhibition	Homo sapiens
3-[[[2-(hydroxyamino)-2-oxo-ethyl]-(4-methoxyphenyl)sulfonylamino]methyl]benzoic acid	hyperbolic noncompetitive/mixed-type inhibition; mixed type inhibition	Homo sapiens
actinonin	mixed type inhibition	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics	Homo sapiens
additional information	-	additional information	enzyme kinetic data for meprin beta suggests hyperbolic v/S characteristics	Homo sapiens
0.074	-	KKGYVADAP-7-amido-4-methylcoumarin	pH 7.5, 30°C, recombinant enzyme	Homo sapiens
0.074	-	KKGYVADAP-7-amido-4-methylcoumarin	pH 7.4, 30°C, recombinant enzyme	Homo sapiens
0.184	-	KKGYVADAP-4-nitroanilide	pH 7.5, 30°C, recombinant enzyme	Homo sapiens
0.184	-	KKGYVADAP-4-nitroanilide	pH 7.4, 30°C, recombinant enzyme	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Homo sapiens	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required	Homo sapiens
Zn2+	required, a zinc-metalloprotease	Homo sapiens
Zn2+	zinc-dependent metalloendoprotease	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	meprin beta preferentially cleaves substrates with acidic amino acids in P1'-position	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q16820	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	the recombinant His6-tagged enzyme is activated by cleavage through trypsin	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged meprin beta from Pichia pastoris strain X33 by nickel affinity chromatography, activation of the enzyme by trypsin, followed by hydrophobic interaction chromatography, and gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
KKGYVADAP-4-nitroanilide + H2O	-	Homo sapiens	KKGYVA + DAP-4-nitroanilide	-	?
KKGYVADAP-7-amido-4-methylcoumarin + H2O	-	Homo sapiens	KKGYVA + DAP-7-amido-4-methylcoumarin	-	?
additional information	meprin beta preferentially cleaves substrates with acidic amino acids in P1'-position	Homo sapiens	?	-	?
additional information	the cleavage of a meprin beta substrate leads to generation of the prolyl tripeptidyl aminopeptidase (PtP, EC 3.4.14.12) substrate, and the activity of PtP results in release of a chromophore or fluorophore, coupled assay method evaluation, overview	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
MEP1B	-	Homo sapiens
meprin A subunit beta	UniProt	Homo sapiens
meprin beta	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4	-	KKGYVADAP-7-amido-4-methylcoumarin	pH 7.5, 30°C, recombinant enzyme	Homo sapiens
4	-	KKGYVADAP-7-amido-4-methylcoumarin	pH 7.4, 30°C, recombinant enzyme	Homo sapiens
20	-	KKGYVADAP-4-nitroanilide	pH 7.5, 30°C, recombinant enzyme	Homo sapiens
20	-	KKGYVADAP-4-nitroanilide	pH 7.4, 30°C, recombinant enzyme	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens
7.5	-	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	inhibition kinetics	Homo sapiens
0.00003	-	3-[[(3-carboxyphenyl)sulfonyl-[2-(hydroxyamino)-2-oxo-ethyl]amino]methyl]benzoic acid	pH 7.5, 30°C, recombinant enzyme	Homo sapiens
0.00003	-	3-[[[2-(hydroxyamino)-2-oxo-ethyl]-(4-methoxyphenyl)sulfonylamino]methyl]benzoic acid	pH 7.5, 30°C, recombinant enzyme	Homo sapiens
0.00003	-	3-[[(3-carboxyphenyl)sulfonyl-[2-(hydroxyamino)-2-oxo-ethyl]amino]methyl]benzoic acid	pH 7.4, 30°C, recombinant enzyme	Homo sapiens
0.00003	-	3-[[[2-(hydroxyamino)-2-oxo-ethyl]-(4-methoxyphenyl)sulfonylamino]methyl]benzoic acid	pH 7.4, 30°C, recombinant enzyme	Homo sapiens
0.0003	-	actinonin	pH 7.5, 30°C, recombinant enzyme	Homo sapiens
0.0003	-	actinonin	pH 7.4, 30°C, recombinant enzyme	Homo sapiens

General Information

General Information	Comment	Organism
evolution	the astacin proteases meprin alpha and meprin beta are zinc-dependent metalloproteases of the metzincin superfamily	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
54.1	-	KKGYVADAP-7-amido-4-methylcoumarin	pH 7.5, 30°C, recombinant enzyme	Homo sapiens
54.1	-	KKGYVADAP-7-amido-4-methylcoumarin	pH 7.4, 30°C, recombinant enzyme	Homo sapiens
108.7	-	KKGYVADAP-4-nitroanilide	pH 7.5, 30°C, recombinant enzyme	Homo sapiens
108.7	-	KKGYVADAP-4-nitroanilide	pH 7.4, 30°C, recombinant enzyme	Homo sapiens

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Release 2023.1 (January 2023)