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Literature summary for 3.4.24.63 extracted from
- Meprin metalloproteases inactivate interleukin 6 (2014), J. Biol. Chem., 289, 7580-7588.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of C-terminally His6-tagged meprin beta protein (1-648) in human HEK-293 cells | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| actinonin | - |
Homo sapiens |  |
| actinonin | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| brush border membrane | - |
Homo sapiens | 31526 | - |
| brush border membrane | - |
Rattus norvegicus | 31526 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required, zinc metalloprotease | Homo sapiens | |
| Zn2+ | required, zinc metalloprotease | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q16820 | - |
- |
| Rattus norvegicus | P28826 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | activation of recombinant zymogen enzyme by trypsin | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged meprin beta protein (1-648) from human HEK-293 cells by metal-chelating affinity chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Homo sapiens | - |
| kidney | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| interleukin-6 + H2O | recombinant human substrate expressed in Escherichia coli, inactivation | Rattus norvegicus | ? | - |
? | |
| interleukin-6 + H2O | recombinant human substrate expressed in eukaryotic B9 cell line, inactivation. Human meprin B cleaves 35% and 65% of human interleukin-6 of about 22 kDa to a smaller product of about 21 kDa within 30 min and 2 h, respectively | Homo sapiens | ? | - |
? | |
| interleukin-6 + H2O | recombinant murine substrate expressed in Escherichia coli, inactivation. Rat meprin B cleaves 65% murine interleukin-6 of about 22 kDa to a smaller product of about 21 kDa within 30 min | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| meprin beta | - |
Homo sapiens |
| meprin beta | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
| 7.5 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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