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Literature summary for 3.4.24.63 extracted from

  • Arnold, P.; Schmidt, F.; Prox, J.; Zunke, F.; Pietrzik, C.; Lucius, R.; Becker-Pauly, C.
    Calcium negatively regulates meprin beta activity and attenuates substrate cleavage (2015), FASEB J., 29, 3549-3557.
    View publication on PubMed
Search for more literature for 3.4.24.63

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of FLAG-tagged enzyme mutants in HEK-293 and COS-7 cells, recombinant expression of N-terminally His-tagged human meprin beta zymogen in Hi5 insect cells via viral transfection method Homo sapiens

Protein Variants

Protein Variants Comment Organism
D240A site-directed mutagenesis, the D204A mutant shows almost no signal at the cell surface, but strongly colocalized with the endoplasmic reticulum marker, low cell surface activity of D204A enzyme Homo sapiens
D245A site-directed mutagenesis Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ calcium negatively regulates meprin beta activity and attenuates substrate cleavage Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ physiologic calcium concentrations display a dual role by (1) directly inhibiting meprin beta activity and (2) supporting meprin beta folding during biosynthesis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amyloid precursor protein + H2O Homo sapiens
-
 ?
-
 ?
dentin phosphoprotein + H2O Homo sapiens
-
 ?
-
 ?
dentin sialophosphoprotein + H2O Homo sapiens
-
 ?
-
 ?
mucin-2 + H2O Homo sapiens
-
 ?
-
 ?
Procollagen + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16820
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein deglycosylation of recombinant enzyme by peptide-N-glycosidase Homo sapiens
proteolytic modification the recombinant N-terminally His-tagged human meprin beta zymogen is activated by cleavage with trypsin Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged human meprin beta zymogen from Hi5 insect cells by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methyloxycoumarin-4-yl)acetyl-EDEDED-NH2-(2,4-dinitrophenyl) + H2O
-
 Homo sapiens ?
-
 ?
amyloid precursor protein + H2O
-
 Homo sapiens ?
-
 ?
amyloid precursor protein + H2O recombinant soluble human APP695, cleavage at the beta-secretase site Homo sapiens ?
-
 ?
dentin phosphoprotein + H2O
-
 Homo sapiens ?
-
 ?
dentin sialophosphoprotein + H2O
-
 Homo sapiens ?
-
 ?
mucin-2 + H2O
-
 Homo sapiens ?
-
 ?
Procollagen + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
meprin beta
-
 Homo sapiens
meprin beta metalloproteinase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
metabolism calcium negatively regulates meprin beta activity and attenuates substrate cleavage Homo sapiens
physiological function meprin beta metalloproteinase is an important enzyme in extracellular matrix turnover, inflammation, and neurodegeneration in humans Homo sapiens