Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Trypsin | peptidase activity of expressed meprin B precursor is absolutely dependent on activation by trypsin | Rattus norvegicus | |
Trypsin | activation is accompanied by reduction in size of 8 kDa | Rattus norvegicus | |
Trypsin | by limited proteolysis | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
rat, expressed in human kidney cell line 293, the expressed protein displays no detectable peptidase activity unless it is activated by removal of amino-terminal prosequence by limited trypsin digestion | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | microvillar membrane, integral membrane protein | Rattus norvegicus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
x * 74000, deglycosylated enzyme, SDS-PAGE under reducing conditions | Rattus norvegicus |
84000 | - |
x * 84000, expressed in human kidney 293 cells, deglycosylated form, SDS-PAGE under reducing conditions | Rattus norvegicus |
97000 | - |
x * 97000, expressed in human kidney 293 cells, SDS-PAGE under reducing conditions | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosidase F-sensitive | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
expressed in human kidney cell line 293, papain-solubilized | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | poor substrate | Rattus norvegicus | ? | - |
? | |
additional information | no substrates are glutaryl-Ala-Ala-Phe 4-methoxynaphthalamine 2-amide | Rattus norvegicus | ? | - |
? | |
Succinyl-Ala-Ala-Ala 4-methylcoumarin 7-amide + H2O | - |
Rattus norvegicus | ? | - |
? | |
Succinyl-Ala-Ala-Val-Ala 4-nitroanilide + H2O | - |
Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 74000, deglycosylated enzyme, SDS-PAGE under reducing conditions | Rattus norvegicus |
oligomer | x * 84000, expressed in human kidney 293 cells, deglycosylated form, SDS-PAGE under reducing conditions | Rattus norvegicus |
oligomer | x * 97000, expressed in human kidney 293 cells, SDS-PAGE under reducing conditions | Rattus norvegicus |