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Literature summary for 3.4.24.63 extracted from

  • Wolz, R.L.; Bond, J.S.
    Meprins A and B (1995), Methods Enzymol., 248, 325-345.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Trypsin
-
Rattus norvegicus
Trypsin only with larger substrates (azocasein or insulin B), not smaller substrates Mus musculus
Trypsin by limited proteolysis (2 h at 37°C, with tosyl-Phe chloromethyl ketone treated trypsin, in 20 mM Tris-HCl buffer, pH 7.5) Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline strong Mus musculus
2-mercaptoethanol
-
Mus musculus
cysteine
-
Mus musculus
EDTA strong Mus musculus
additional information no inhibition by captopril, PMSF, pepstatin; phosphoramidon, iodoacetic acid, 3,4-dichloroisocoumarin or soybean trypsin inhibitor Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Rattus norvegicus 16020
-
membrane renal brush-border membrane Mus musculus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metalloendopeptidase according to inhibition profile Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid composition and secondary structure similar to meprin A Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
more than 20 inbred, recombinant and congenic strains
-
Mus musculus
-
inbred strain C3H/He
-
Mus musculus
-
male adults
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
C3H/He mouse Mus musculus
papain-solubilized Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
small intestine
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O poor substrate Mus musculus ?
-
?
azocasein + H2O poor substrate Homo sapiens ?
-
?
azocasein + H2O poor substrate Rattus norvegicus ?
-
?
azocasein + H2O mouse enzyme: poor substrate unless activated by trypsin treatment (i.e. latent azocaseinase activity) Mus musculus ?
-
?
Insulin B-chain + H2O 4 cleavage sites (2 major and 2 minor): His5-Leu6, Leu6-Cys(SO3-)7, Ala14-Leu15, Cys(SO3-)19-Gly20 Mus musculus ?
-
?
Insulin B-chain + H2O mouse enzyme Mus musculus ?
-
?
additional information no substrates are nitrobradykinin Mus musculus ?
-
?
additional information no substrates are nitrobradykinin Homo sapiens ?
-
?
additional information no substrates are nitrobradykinin Rattus norvegicus ?
-
?
additional information member of astacin family Mus musculus ?
-
?
additional information member of astacin family Homo sapiens ?
-
?
additional information member of astacin family Rattus norvegicus ?
-
?
N-Benzoyl-Tyr 4-aminobenzoate + H2O rat or human enzyme, cleaves arylamide bond Homo sapiens ?
-
?
N-Benzoyl-Tyr 4-aminobenzoate + H2O rat or human enzyme, cleaves arylamide bond Rattus norvegicus ?
-
?
Tyr-Leu-Val-Cys(SO3-)-Gly-Glu-Arg-Gly + H2O synthetic octapeptide, derived from insulin B-chain Mus musculus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58
-
t1/2: 2.5 min, less stable than meprin A Mus musculus