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Literature summary for 3.4.24.60 extracted from

  • Clamagirand, C.; Joudiou, C.; Cohen, P.
    Dactylysin (2004), Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 1042-1044.
No PubMed abstract available
Search for more literature for 3.4.24.60

Inhibitors

Inhibitors Comment Organism Structure
(Leu5)-enkephalin inhibitor of dactylysin towards substance P Xenopus laevis
(Met5)-enkephalin inhibitor of dactylysin towards substance P Xenopus laevis
1,10-phenanthroline strongly inhibits Xenopus laevis
DTT
-
 Xenopus laevis
EDTA strongly inhibits Xenopus laevis
EGTA strongly inhibits Xenopus laevis
additional information not inhibited by TIMPs and 1mM captopril Xenopus laevis
phosphoramidon
-
 Xenopus laevis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 (Arg0,Leu6)-enkephalin
-
 Xenopus laevis
0.018
-
 Somatostatin-14
-
 Xenopus laevis
0.02
-
 Substance P
-
 Xenopus laevis
0.028
-
 antibacterial peptide PGLa
-
 Xenopus laevis
0.041
-
 bradykinin
-
 Xenopus laevis
0.05
-
 dynorphin A
-
 Xenopus laevis
0.05
-
 atrial natriuretic factor
-
 Xenopus laevis
0.063
-
 angiotensin II
-
 Xenopus laevis
0.079
-
 (Leu5,Arg6)-enkephalin
-
 Xenopus laevis
0.125
-
 Neurokinin A
-
 Xenopus laevis
0.125
-
 beta-amyloid derived peptide
-
 Xenopus laevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
 subunit that retains the proteolytic activity Xenopus laevis
90000 100000
-
 Xenopus laevis

Organism

Organism UniProt Comment Textmining
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2000fold purified by a four-step fractionation including successively ion-exchange column, hydrophobic chromatographies and gel filtration Xenopus laevis

Source Tissue

Source Tissue Comment Organism Textmining
gland
-
 Xenopus laevis
-
skin
-
 Xenopus laevis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Arg0,Leu6)-enkephalin + H2O cleavage of Phe4-Leu5 Xenopus laevis ?
-
 ?
(Leu5,Arg6)-enkephalin + H2O cleavage of Phe4-Leu5 Xenopus laevis YGGF + Leu-Arg
-
 ?
angiotensin II + H2O cleavage of Tyr4-Ile5 Xenopus laevis ?
-
 ?
Antibacterial peptide PGLa + H2O
-
 Xenopus laevis ?
-
 ?
atrial natriuretic factor + H2O cleavage of Ser-Phe Xenopus laevis LGCNS + Phe-Arg-Tyr
-
 ?
beta-amyloid derived peptide + H2O cleavage of Gly-Leu Xenopus laevis ?
-
 ?
bradykinin + H2O cleavage of Gly4-Phe5 Xenopus laevis ?
-
 ?
dynorphin A + H2O
-
 Xenopus laevis ?
-
 ?
dynorphin A1-16 + H2O cleavage of Phe-Leu Xenopus laevis ?
-
 ?
kermit + H2O cleavage of the peptide link Ser-Phe Xenopus laevis ?
-
 ?
additional information no cleavage of (Leu5)-enkephalin and (Met5)-enkephalin Xenopus laevis ?
-
 ?
Neurokinin A + H2O cleavage of Gly-Leu Xenopus laevis ?
-
 ?
neurokinin A + H2O
-
 Xenopus laevis HKTDSFVG + Leu-Met-NH2
-
 ?
peptide PglA + H2O antimicrobial peptide of Xenopus laevis skin secretion predominantly cleaved at the Lys12-Ile13 bond Xenopus laevis ?
-
 ?
Somatostatin-14 + H2O cleavage of Phe-Phe in the intersulfide bridge motif Xenopus laevis ?
-
 ?
substance P + H2O cleavage of Gly9-Leu10 Xenopus laevis RPKQQF + Phe-Gly + Leu-Met-NH2
-
 ?

Synonyms

Synonyms Comment Organism
peptide hormone inactivating enzyme
-
 Xenopus laevis
PHIE
-
 Xenopus laevis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0001
-
 acid-pretreated corn stover 41% inhibition Xenopus laevis
0.0001
-
 1,10-phenanthroline 98% inhibition Xenopus laevis
0.001
-
 DTT 70% inhibition when tested on kermit Xenopus laevis