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Literature summary for 3.4.24.60 extracted from

  • Delporte, C.; Carvalho, K.M.; Leseney, A.M.; Winand, J.; Christophe, J.; Cohen, P.
    A new metallo- endopeptidase from human neuroblastoma NB-OK-1 cells which inactivates atrial natriuretic peptide by selective cleavage at the Ser123-Phe124 bond (1992), Biochem. Biophys. Res. Commun., 182, 158-164.
    View publication on PubMed
Search for more literature for 3.4.24.60

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline metalloendopeptidase character Homo sapiens
benzamidine weak Homo sapiens
EDTA metalloendopeptidase character Homo sapiens
EGTA metalloendopeptidase character Homo sapiens
additional information no inhibition by N-tosyl-L-Phe chloromethyl ketone (i.e. TPCK); pepstatin, guanidylethylmercaptosuccinic acid (i.e. GEMSA), captopril, aprotinin, soybean trypsin inhibitor (i.e. STI), PMSF, IAA Homo sapiens
N-ethylmaleimide
-
 Homo sapiens
p-chloromercuriphenylsulfonic acid
-
 Homo sapiens
phosphoramidon not Homo sapiens
thiorphan not Homo sapiens
tosyl-Arg methyl ester i.e. TAME, weak Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 atrial natriuretic peptide
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metalloendopeptidase Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
neuroblastoma cell line NB-OK-1 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin II + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe, cleavage site: Tyr4-Ile5 Homo sapiens ?
-
 ?
ANP-(103-126) + H2O i.e. atrial natriuretic peptide, cleavage sites: Xaa-Phe, Xaa-Leu or Xaa-Ile bonds Homo sapiens ANP-(103-123) + Phe124-Arg125-Tyr126
-
 ?
Asp-Ala-Asp-Thr-Cys-[D-Arg8]-kermit + H2O model substrate, derivative of kermit, cleavage site: Ser12-Phe13 Homo sapiens ?
-
 ?
atrial natriuretic peptide + H2O cleavage at Ser123-Phe124 Homo sapiens ?
-
 ?
bradykinin + H2O i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg, major cleavage site: Gly4-Phe5, minor site: Pro7-Pro8 Homo sapiens ?
-
 ?
Litorin + H2O minor sites: Gly6-His7 Homo sapiens ?
-
 ?
Litorin + H2O major cleavage sites: His7-Phe8 Homo sapiens ?
-
 ?
additional information no cleavage of analogous bonds in [Leu5]-enkephalin, oxytocin, vasopressin Homo sapiens ?
-
 ?
additional information cleavage sites: Xaa-Phe, Xaa-Leu, Xaa-Ile Homo sapiens ?
-
 ?
Neuromedin C + H2O Gly-Asn-His-Trp-Ala-Val-Gly-His-Leu-Met-NH2, major cleavage site: His8-Leu9, minor site: Gly7-His8 Homo sapiens ?
-
 ?
Somatostatin-14 + H2O i.e. Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys, cleavage site: Phe6-Phe7 within disulfide bridged segment yielding one single product Homo sapiens ?
-
 ?
Substance P + H2O major cleavage site: Gly9-Leu10, minor site: Phe7-Phe8 Homo sapiens ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Homo sapiens