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Literature summary for 3.4.24.57 extracted from
- O-Sialoglycoprotease from Pasteurella haemolytica (1995), Methods Enzymol., 248, 728-740.
General Stability
| General Stability | Organism |
|---|---|
| Dilute solutions lose activity at 0-4°C, or on repeated freezing and thawing | Mannheimia haemolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| high salt concentration | irreversible, 0.3 M NaCl inactivates within a few h | Mannheimia haemolytica | |
| additional information | not: phosphoramidon | Mannheimia haemolytica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
Pasteurella haemolytica, calculation from amino acid sequence | Mannheimia haemolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mannheimia haemolytica | - |
A1 | - |
| Mannheimia haemolytica A1 | - |
A1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Mannheimia haemolytica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Mannheimia haemolytica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mannheimia haemolytica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or -12°C, stable for over 1 year | Mannheimia haemolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Cell-surface O-sialoglycoproteins + H2O | no activity with glycoproteins which contain N-linked glycans but no O-sialo-glycans, leukocyte integrins, transferrin receptor, HLA class I and 8A3 Ag | Mannheimia haemolytica | ? | - |
? |  |
| Cell-surface O-sialoglycoproteins + H2O | CD34, CD43 (leukosialin), CD44 (hyaluronic acid receptor) and CD45 (leukocyte common Ag) | Mannheimia haemolytica | ? | - |
? | |
| Cell-surface O-sialoglycoproteins + H2O | no activity with glycoproteins which contain N-linked glycans but no O-sialo-glycans, leukocyte integrins, transferrin receptor, HLA class I and 8A3 Ag | Mannheimia haemolytica A1 | ? | - |
? | |
| Cell-surface O-sialoglycoproteins + H2O | CD34, CD43 (leukosialin), CD44 (hyaluronic acid receptor) and CD45 (leukocyte common Ag) | Mannheimia haemolytica A1 | ? | - |
? | |
| Glycophorin A + H2O | - |
Mannheimia haemolytica | ? | - |
? | |
| Glycophorin A + H2O | - |
Mannheimia haemolytica A1 | ? | - |
? | |
| additional information | not: furylacryloyl-Gly-Leu amide | Mannheimia haemolytica | ? | - |
? | |
| additional information | not: furylacryloyl-Gly-Leu amide | Mannheimia haemolytica A1 | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Mannheimia haemolytica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | stable | Mannheimia haemolytica |
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Release 2023.1 (January 2023)
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