Literature summary for 3.4.24.56 extracted from

Ivancic, V.A.; Krasinski, C.A.; Zheng, Q.; Meservier, R.J.; Spratt, D.E.; Lazo, N.D.
Enzyme kinetics from circular dichroism of insulin reveals mechanistic insights into the regulation of insulin-degrading enzyme (2018), Biosci. Rep., 38, BSR20181416 .

Search for more literature for 3.4.24.56

Cloned(Commentary)

Cloned (Comment)	Organism
wild-type and E111Q glutathione S-transferase tagged human insulin-degrading enzyme are expressed in Escherichia coli BL21 (DE3) codon plus competent cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E111Q	inactive enzyme form	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.02	-	Insulin	pH 7.4, 37°C	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	zinc metalloprotease	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	the enzyme selectively degrades biologically important substrates associated with type 2 diabetes and Alzheimer's disease	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P14735	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
insulin + H2O	-	Homo sapiens	?	-	?
additional information	the enzyme selectively degrades biologically important substrates associated with type 2 diabetes and Alzheimer's disease	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
IDE	-	Homo sapiens
Insulin-degrading enzyme	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.048	-	Insulin	pH 7.4, 37°C	Homo sapiens

General Information

General Information	Comment	Organism
metabolism	ATP regulates insulin-degrading enzyme-dependent degradation of insulin, but the addition of Mg2+ abolishes the regulation	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.4	-	Insulin	pH 7.4, 37°C	Homo sapiens

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Release 2023.1 (January 2023)