Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M sodium cacodylate (pH 6.5), 0.2 M MgCl2, and 10% (w/v) PEG-3000 at 18°C | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F530A | the mutation renders the enzyme hyperactive, with up to a 20fold enhancement in degrading activity | Homo sapiens |
G361A/G362A | the mutant has reduced enzymatic activity | Homo sapiens |
P284G | the mutation results in a slight reduction of enzyme catalysis | Homo sapiens |
P286G | the mutation results in a significant reduction of enzyme catalysis | Homo sapiens |
P289G | the mutant shows wild type activity | Homo sapiens |
P292G | the mutation results in intermediate reduction of enzyme catalysis | Homo sapiens |
R767A | the mutant exists mostly as a monomer | Homo sapiens |
S132C/E817C | the mutant preferentially stays in the closed state | Homo sapiens |
Y496A | the mutation dramatically impairs the enzymatic activity | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains zinc | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amyloid beta-peptide + H2O | Homo sapiens | - |
? | - |
? | |
insulin + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14735 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Source Q column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methoxycoumarin-4-yl-acetyl-RPPGF-SAFK-2,4-dinitrophenyl + H2O | - |
Homo sapiens | ? | - |
? | |
amyloid beta-peptide + H2O | - |
Homo sapiens | ? | - |
? | |
insulin + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | X-ray scattering | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
IDE | - |
Homo sapiens |
Insulin-degrading enzyme | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | insulin-degrading enzyme selectively degrades the monomer of amyloidogenic peptides and contributes to clearance of amyloid-beta. Thus, the enzyme retards the progression of Alzheimers disease | Homo sapiens |