Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | 40fold activation for wild-type | Rattus norvegicus | |
bradykinin | 4fold activation for wild-type | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expressed as hexahistidine fusion proteins in SF9 insect cell | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
E111F | crystal structure of a catalytically compromised E111F mutant of IDE has electron density for peptide ligands bound at the active site in domain 1 and a distal site in domain 2 | Rattus norvegicus |
I374S | mutation in the distal site eliminates allosterism, Vmax decreased approximately 10fold compared to wild-type, Km (Abz-GGFLRKHGQ-EDDnp) roughly similar to wild-type, no heterotropic activation with bradykinin, no significant activation through ATP | Rattus norvegicus |
V360S | mutation in the distal site eliminates allosterism, Vmax decreased approximately 10fold compared to wild-type, Km (Abz-GGFLRKHGQ-EDDnp) roughly similar to wild-type, no heterotropic activation with bradykinin, ATP activation is reduced to 8fold | Rattus norvegicus |
Y609F | mutation in the distal site eliminates allosterism, Vmax decreased approximately 10fold compared to wild-type, Km (Abz-GGFLRKHGQ-EDDnp) roughly similar to wild-type, no heterotropic activation with bradykinin, no significant activation through ATP | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0068 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.4, temperature not specified in the publication, mutant T609F, Vmax: 2.6 nmol/min/mg | Rattus norvegicus | |
0.0085 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.4, temperature not specified in the publication, mutant I374S, Vmax: 3.45 nmol/min/mg | Rattus norvegicus | |
0.00861 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.4, temperature not specified in the publication, mutant V360S, Vmax: 2 nmol/min/mg | Rattus norvegicus | |
0.01587 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.4, temperature not specified in the publication, wild-type, Vmax: 35.5 nmol/min/mg | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-GGFLRKHGQ-EDDnp + H2O | - |
Rattus norvegicus | Abz-GGFLR + KHGQ-EDDnp | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
IDE | - |
Rattus norvegicus |
Insulysin | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |