Cloned (Comment) | Organism |
---|---|
recombinant expression of His6-tagged IDE in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
E768A | naturally occuring mutation of IDE | Rattus norvegicus |
Y248C | naturally occuring mutation of IDE | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Rattus norvegicus | |
2-mercaptoethanol | - |
Rattus norvegicus | |
4,7-phenanthroline | - |
Rattus norvegicus | |
cysteine | - |
Rattus norvegicus | |
DTT | - |
Rattus norvegicus | |
EDTA | - |
Rattus norvegicus | |
Insulin | substrate inhibition | Rattus norvegicus | |
additional information | IDE is inhibited by metal chelators, thiol modifiers, inhibitors of cysteine protease activity and insulin, no inhibition by GTP and DMSO, poor inhibition by ATP | Rattus norvegicus | |
NEM | - |
Rattus norvegicus | |
PMSF | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.219 | - |
Abz-SEKKDNYIIKGV-nitroY-OH | pH 9.2, 37°C, recombinant enzyme | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amyloid beta40 + H2O | Rattus norvegicus | - |
amyloid beta40 peptide fragments | - |
? | |
insulin + H2O | Rattus norvegicus | cleavage of the B-chain | insulin peptide fragments | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged IDE from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.22 | - |
pH 9.2, 37°C, purified recombinant His-tagged enzyme, substrate Abz-SEKKDNYIIKGV-nitroY-OH | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-GFLRKGVQ-EDDnp + H2O | - |
Rattus norvegicus | ? | - |
? | |
Abz-SEKKDNYIIKGV-nitroY-OH + H2O | a substrate based on the polypeptide sequence of the yeast P2 a-factor mating propheromone | Rattus norvegicus | ? | - |
? | |
amyloid beta40 + H2O | - |
Rattus norvegicus | amyloid beta40 peptide fragments | - |
? | |
insulin + H2O | cleavage of the B-chain | Rattus norvegicus | insulin peptide fragments | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDE | - |
Rattus norvegicus |
Insulin-degrading enzyme | - |
Rattus norvegicus |
More | IDE belongs to the M16A enzyme family | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 49 | temperature profile, recombinant enzyme, overview | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 9.2 | pH profile, recombinant enzyme, overview | Rattus norvegicus |