Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.56 extracted from

  • Manolopoulou, M.; Guo, Q.; Malito, E.; Schilling, A.B.; Tang, W.J.
    Molecular basis of catalytic chamber-assisted unfolding and cleavage of human insulin by human insulin-degrading enzyme (2009), J. Biol. Chem., 284, 14177-14188.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged IDE in Escherichia coli strain Rosetta (DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant mutant E110Q with bound insulin, hanging drop vapor diffusion method, 0.001 ml of 16-20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 50 mM NaCl, is mixed with 0.001 ml of reservoir solution containing, 10-13% PEG MME 5000, 100 mM HEPES, pH 7.0, 4-14% tacsimate, and 10% dioxane, equilibration over 0.5 ml of reservoir solution, 18°C, 3-5 days, X-ray diffraction structure determination and analysis at 2.6-2.8 A resolution, molecular replacement Homo sapiens

Protein Variants

Protein Variants Comment Organism
E110Q structure comparison to wild-type enzyme structure Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
insulin + H2O Homo sapiens
-
insulin peptide fragments
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged IDE from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
Degradation of insulin, glucagon and other polypeptides. No action on proteins molecular basis of catalytic chamber-assisted unfolding and cleavage of human insulin by human insulin-degrading enzyme, IDE utilizes the interaction of its exosite with the N-terminus of the insulin A chain, overview. The unique size, shape, charge distribution, and exosite of the IDE catalytic chamber contribute to its high affinity for insulin Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methoxycoumarin-4-yl-acetyl-RPPGFSAFK-2,4-dinitrophenyl + H2O fluorogenic bradykinin-mimetic IDE substrate V Homo sapiens ?
-
?
insulin + H2O
-
Homo sapiens insulin peptide fragments
-
?
insulin + H2O IDE forms an enclosed catalytic chamber that completely engulfs and intimately interacts with a partially unfolded insulin molecule. The unique size, shape, charge distribution, and exosite of the IDE catalytic chamber contribute to its high affinity for insulin, IDE-insulin binding structure and interaction analysis, overview Homo sapiens insulin peptide fragments
-
?

Synonyms

Synonyms Comment Organism
IDE
-
Homo sapiens
Insulin-degrading enzyme
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Homo sapiens