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Literature summary for 3.4.24.56 extracted from

  • Ciaccio, C.; Tundo, G.R.; Grasso, G.; Spoto, G.; Marasco, D.; Ruvo, M.; Gioia, M.; Rizzarelli, E.; Coletta, M.
    Somatostatin: a novel substrate and a modulator of insulin-degrading enzyme activity (2009), J. Mol. Biol., 385, 1556-1567.
    View publication on PubMed
Search for more literature for 3.4.24.56

Activating Compound

Activating Compound Comment Organism Structure
Somatostatin somatostatin binding to IDE brings about a concentration-dependent structural change of the secondary and tertiary structure of the enzyme, revealing two possible binding sites. The higher affinity binding site disappears upon inactivation of IDE by ethylenediaminetetraacetic acid, which chelates the catalytic Zn2+ ion Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA the activation of IDE disappears upon inactivation by EDTA, which chelates the catalytic Zn2+ ion Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 Dabcyl-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Glu(EDANS)-NH2 pH 7.3, 37°C, presence of 0.04 mM somatostatin Homo sapiens
2.3
-
 Dabcyl-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Glu(EDANS)-NH2 pH 7.3, 37°C Homo sapiens
2.5
-
 amyloid beta1-40 pH 7.3, 37°C Homo sapiens
7.5
-
 Somatostatin pH 7.3, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amyloid beta1-40 + H2O
-
 Homo sapiens ?
-
 ?
Dabcyl-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Glu(EDANS)-NH2 + H2O fluorogenic derivative of amyloid beta containing residues 10-25 Homo sapiens ?
-
 ?
somatostatin + H2O cleavage at Phe6-Phe7 bond Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
 Somatostatin pH 7.3, 37°C Homo sapiens
8
-
 amyloid beta1-40 pH 7.3, 37°C Homo sapiens
61
-
 Dabcyl-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Glu(EDANS)-NH2 pH 7.3, 37°C, presence of 0.04 mM somatostatin Homo sapiens
62.7
-
 Dabcyl-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Glu(EDANS)-NH2 pH 7.3, 37°C Homo sapiens