Cloned (Comment) | Organism |
---|---|
a form of the enzyme derived from an alternative translational start site that can localize to mitochondria instead of the cytosol | Mus musculus |
a form of the enzyme derived from an alternative translational start site that can localize to mitochondria instead of the cytosol | Homo sapiens |
a form of the enzyme derived from an alternative translational start site that can localize to mitochondria instead of the cytosol | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
H18R/A890V | naturally occuring mutations in the insulysin gene in GK rats causing 30% reduced enzyme activity and type 2 diabetes as well as increased amyloid beta peptide levels , the rats exhibit defects in both insulin action and insulin degradation, the increased amyloid beta-peptide levels do not lead to increased steady-state levels of its activity due to compensatory degradative mechanisms in the brain | Rattus norvegicus |
additional information | overexpression of insulysin in human amyloid precursor protein transgenic mice leads to a decrease in amyloid beta peptide levels | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Homo sapiens | 9986 | - |
cytosol | primarily | Mus musculus | 5829 | - |
cytosol | primarily | Homo sapiens | 5829 | - |
cytosol | primarily | Rattus norvegicus | 5829 | - |
mitochondrion | a form of the enzyme derived from an alternative translational start site that can localize to mitochondria | Mus musculus | 5739 | - |
mitochondrion | a form of the enzyme derived from an alternative translational start site that can localize to mitochondria | Homo sapiens | 5739 | - |
mitochondrion | a form of the enzyme derived from an alternative translational start site that can localize to mitochondria | Rattus norvegicus | 5739 | - |
peroxisome | - |
Mus musculus | 5777 | - |
peroxisome | - |
Homo sapiens | 5777 | - |
peroxisome | - |
Rattus norvegicus | 5777 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | one Zn2+ bound at the active site, with the zinc-binding motif HXXEH | Mus musculus | |
Zn2+ | one Zn2+ bound at the active site, with the zinc-binding motif HXXEH | Homo sapiens | |
Zn2+ | one Zn2+ bound at the active site, with the zinc-binding motif HXXEH | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
about | Mus musculus |
110000 | - |
about | Homo sapiens |
110000 | - |
about | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amyloid beta-peptide + H2O | Mus musculus | degradation, role for insulysin in regulating amyloid beta peptide levels in the brain | ? | - |
? | |
amyloid beta-peptide + H2O | Homo sapiens | degradation, role for insulysin in regulating amyloid beta peptide levels in the brain | ? | - |
? | |
amyloid beta-peptide + H2O | Rattus norvegicus | degradation, role for insulysin in regulating amyloid beta peptide levels in the brain | ? | - |
? | |
insulin + H2O | Mus musculus | degradation, insulin internalized into Hep-G2 cells is able cross-link with intracellular insulysin | ? | - |
? | |
insulin + H2O | Homo sapiens | degradation, insulin internalized into Hep-G2 cells is able cross-link with intracellular insulysin | ? | - |
? | |
insulin + H2O | Rattus norvegicus | degradation, type 2 diabetic GK rats exhibit defects in both insulin action and insulin degradation mainly due to mutation H18R and A890V in the insulysin protein | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
healthy and type 2 diabetic GK rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
brain | - |
Homo sapiens | - |
brain | - |
Rattus norvegicus | - |
endothelial cell | cerebrovascular, the this cell surface form is the major form of insulysin responsible for degrading amyloid beta-peptides | Homo sapiens | - |
Hep-G2 cell | - |
Homo sapiens | - |
hepatocyte | primary | Homo sapiens | - |
hippocampus | insulysin is reduced in the hippocampus of Alzheimer's disease patients with an apolipoprotein E-4 allele | Homo sapiens | - |
lymphocyte | - |
Homo sapiens | - |
additional information | insulysin activity declines with aging in the hippocampus of a mouse model of Alzheimers disease | Mus musculus | - |
neuron | primary cell culture | Rattus norvegicus | - |
pancreatic acinar cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amyloid beta-peptide + H2O | degradation | Mus musculus | ? | - |
? | |
amyloid beta-peptide + H2O | degradation | Homo sapiens | ? | - |
? | |
amyloid beta-peptide + H2O | degradation | Rattus norvegicus | ? | - |
? | |
amyloid beta-peptide + H2O | degradation, role for insulysin in regulating amyloid beta peptide levels in the brain | Mus musculus | ? | - |
? | |
amyloid beta-peptide + H2O | degradation, role for insulysin in regulating amyloid beta peptide levels in the brain | Homo sapiens | ? | - |
? | |
amyloid beta-peptide + H2O | degradation, role for insulysin in regulating amyloid beta peptide levels in the brain | Rattus norvegicus | ? | - |
? | |
insulin + H2O | degradation | Mus musculus | ? | - |
? | |
insulin + H2O | degradation | Homo sapiens | ? | - |
? | |
insulin + H2O | degradation | Rattus norvegicus | ? | - |
? | |
insulin + H2O | degradation, insulin internalized into Hep-G2 cells is able cross-link with intracellular insulysin | Mus musculus | ? | - |
? | |
insulin + H2O | degradation, insulin internalized into Hep-G2 cells is able cross-link with intracellular insulysin | Homo sapiens | ? | - |
? | |
insulin + H2O | degradation, type 2 diabetic GK rats exhibit defects in both insulin action and insulin degradation mainly due to mutation H18R and A890V in the insulysin protein | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 110000, about | Mus musculus |
monomer | 1 * 110000, about | Homo sapiens |
monomer | 1 * 110000, about | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
IDE | - |
Mus musculus |
IDE | - |
Homo sapiens |
IDE | - |
Rattus norvegicus |
insulin degrading enzyme | - |
Mus musculus |
insulin degrading enzyme | - |
Homo sapiens |
insulin degrading enzyme | - |
Rattus norvegicus |