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Literature summary for 3.4.24.50 extracted from

  • Tanizaki, M.M.; Zingali, R.B.; Kawazaki, H.; Imajoh, S.; Yamazaki, S.; Suzuki, K.
    Purification and some characteristics of a zinc metalloprotease from the venom of Bothrops jararaca (jararaca) (1989), Toxicon, 27, 747-755.
    View publication on PubMed
Search for more literature for 3.4.24.50

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline + 4 mM Ca2+: 85% inhibition Bothrops jararaca
5,5'-dithiobis(2-nitrobenzoic acid) 1 mM: 70% inhibition, in presence of 2 mM Ca2+: 30% inhibition Bothrops jararaca
EDTA 0.1 mM: complete inhibition Bothrops jararaca
additional information phosphoramidon, leupeptin, epoxysuccinyl derivatives e.g. E-64, Ep-475 or Ep-479, all 0.5 mM, are no inhibitors Bothrops jararaca

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Bothrops jararaca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 1 * 23000, Bothrops jararaca, SDS-PAGE Bothrops jararaca

Organism

Organism UniProt Comment Textmining
Bothrops jararaca
-
 jararaca snake
-

Purification (Commentary)

Purification (Comment) Organism
partial Bothrops jararaca

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Bothrops jararaca
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O cleavage of Pro-Phe Bothrops jararaca Asp-Arg-Val-Tyr-Ile-His-Pro + Phe-His-Leu
-
 ?
Insulin B-chain + H2O cleavage of Gln4-His, Ser9-His and Ala14-Leu Bothrops jararaca Hydrolyzed insulin B-chain
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 23000, Bothrops jararaca, SDS-PAGE Bothrops jararaca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bothrops jararaca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 assay at Bothrops jararaca