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Literature summary for 3.4.24.49 extracted from
- Isolation and characterization of a proteolytic enzyme from the venom of the snake Bothrops jararaca (Jararaca) (1982), Toxicon, 20, 955-972.
General Stability
| General Stability | Organism |
|---|---|
| Ca2+ stabilizes | Bothrops jararaca |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete inhibition | Bothrops jararaca |  |
| EGTA | complete inhibition | Bothrops jararaca | |
| oxalate | 85% inhibition, restorage of 85% of activity by removal of oxalate by addition of Ca2+ | Bothrops jararaca | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM required for maximal activity | Bothrops jararaca | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37300 | - |
Bothrops jararaca, sedimentation equilibrium centrifugation in presence of EDTA | Bothrops jararaca |
| 48000 | - |
x * 37300, Bothrops jararaca, SDS-PAGE in presence of EDTA, x * 48000, Bothrops jararaca, SDS-PAGE | Bothrops jararaca |
| 49870 | - |
Bothrops jararaca, sedimentation equilibrium centrifugation in presence of 6 M guanidine-HCl and 0.1 M 2-mercaptoethanol | Bothrops jararaca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bothrops jararaca | - |
jararaca snake | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bothrops jararaca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Bothrops jararaca | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | endopeptidase | Bothrops jararaca | hydrolyzed casein | - |
? | |
| Insulin B-chain + H2O | cleavage of His5-Leu, His10-Leu, Ala14-Leu, Tyr16-Leu and Phe24-Phe | Bothrops jararaca | Hydrolyzed insulin B-chain | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | x * 37300, Bothrops jararaca, SDS-PAGE in presence of EDTA, x * 48000, Bothrops jararaca, SDS-PAGE | Bothrops jararaca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Bothrops jararaca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Bothrops jararaca |
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