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Literature summary for 3.4.24.48 extracted from

  • Mori, N.; Nikali, T.; Sugihara, H.; Tu, A.T.
    Biochemical characterization of hemorrhagic toxins with fibrinogenase activity isolated from Crotalus ruber ruber venom (1987), Arch. Biochem. Biophys., 253, 108-121.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Crotalus ruber ruber
EDTA
-
Crotalus ruber ruber
additional information no inhibition by benzamidine, tosyl-L-Phe chloromethyl ketone, soybean or egg white trypsin inhibitor or PCMB Crotalus ruber ruber

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ metalloproteinase, contains 1.1 mol Ca2+/mol enzyme Crotalus ruber ruber
additional information less than 0.3 mol Mg2+/mol enzyme and less than 0.9 mol K+/mol enzyme Crotalus ruber ruber
Na+ metalloproteinase, contains 10.3 mol Na+/mol enzyme Crotalus ruber ruber
Zn2+ metalloproteinase, contains 1.1 mol Zn2+/mol enzyme Crotalus ruber ruber

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid composition of isozyme HT-2 Crotalus ruber ruber
25000
-
x * 25000, Crotalus ruber ruber, isozyme HT-2, SDS-PAGE Crotalus ruber ruber

Organism

Organism UniProt Comment Textmining
Crotalus ruber ruber
-
red rattlesnake
-

Purification (Commentary)

Purification (Comment) Organism
from lyophilized crude venom Crotalus ruber ruber

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Crotalus ruber ruber
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu, cleaved at His-Pro and Pro-Phe Crotalus ruber ruber ?
-
?
casein + H2O
-
Crotalus ruber ruber ?
-
?
Dimethylcasein + H2O
-
Crotalus ruber ruber ?
-
?
Fibrin + H2O
-
Crotalus ruber ruber ?
-
?
Fibrinogen + H2O Aalpha chain, not beta or gamma chain Crotalus ruber ruber ?
-
?
hide powder azure + H2O
-
Crotalus ruber ruber ?
-
?
Luteinizing hormone-releasing hormone + H2O cleavage sites: Trp3-Ser4, Gly6-Leu7 Crotalus ruber ruber ?
-
?
Met-enkephalin + H2O i.e. Tyr-Gly-Gly-Phe-Met, cleavage site: Gly3-Phe4 Crotalus ruber ruber Tyr-Gly-Gly + Phe-Met
-
?
additional information exhibits proteolytic, hemorrhatic and myonecrotic activity Crotalus ruber ruber ?
-
?
Oxidized insulin B-chain + H2O cleavage sites: His10-Leu11, Ala14-Leu15, Tyr16-Leu17, Gly23-Phe24 Crotalus ruber ruber Hydrolyzed insulin B-chain
-
?

Subunits

Subunits Comment Organism
? x * 25000, Crotalus ruber ruber, isozyme HT-2, SDS-PAGE Crotalus ruber ruber

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 5.2 Crotalus ruber ruber