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Literature summary for 3.4.24.47 extracted from
- Isolation and characterization of a hemorrhagic proteinase from timber rattlesnake venom (1983), Biochemistry, 22, 749-755.
General Stability
| General Stability | Organism |
|---|---|
| Extensive dialysis against distilled water inactivates, incubation with Zn2+, Ca2+ or Mg2+ fails to reconstitute activity | Crotalus horridus horridus |
| Stable to dialysis against 0.2 M Tris buffer, pH 7.5 | Crotalus horridus horridus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Crotalus horridus horridus |  |
| dithiothreitol | - |
Crotalus horridus horridus | |
| EDTA | - |
Crotalus horridus horridus | |
| additional information | no inhibition by alpha1-antitrypsin, N-ethylmaleimide, iodoacetamide, phenylmethylsulfonylfluoride, egg white or soybean trypsin inhibitor | Crotalus horridus horridus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | zinc and calcium containing metalloproteinase, no activation by added Ca2+ | Crotalus horridus horridus | |
| Mg2+ | 0.14 mol/mol enzyme | Crotalus horridus horridus | |
| Zn2+ | 1 mol zinc/mol enzyme | Crotalus horridus horridus | |
| Zn2+ | zinc and calcium containing metalloproteinase | Crotalus horridus horridus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition compared to other rattlesnake venom proteins | Crotalus horridus horridus |
| 52000 | - |
Crotalus horridus horridus, gel filtration | Crotalus horridus horridus |
| 56000 | - |
1 * 56000, Crotalus horridus horridus, SDS-PAGE | Crotalus horridus horridus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Crotalus horridus horridus | hemorrhage inducing toxin | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crotalus horridus horridus | - |
Timber rattlesnake | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | no glycoprotein | Crotalus horridus horridus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Crotalus horridus horridus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Crotalus horridus horridus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in either lyophilized or frozen state, at least 3 years | Crotalus horridus horridus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | denatured casein, poor substrate | Crotalus horridus horridus | ? | - |
? | |
| Hemoglobin + H2O | poor substrate | Crotalus horridus horridus | ? | - |
? | |
| hide powder azure + H2O | - |
Crotalus horridus horridus | ? | - |
? | |
| additional information | - |
Crotalus horridus horridus | ? | - |
? | |
| additional information | hemorrhage inducing toxin | Crotalus horridus horridus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 56000, Crotalus horridus horridus, SDS-PAGE | Crotalus horridus horridus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Crotalus horridus horridus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.1 | Crotalus horridus horridus |
| 7.5 | - |
hide powder azure as substrate, 0.2 M Tris buffer | Crotalus horridus horridus |
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Release 2023.1 (January 2023)
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