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Literature summary for 3.4.24.44 extracted from

  • Fox, J.W.; Bjarnason, J.B.
    Atrolysins: metalloproteinases from Crotalus atrox venom (1995), Methods Enzymol., 248, 368-387.
    View publication on PubMed
Search for more literature for 3.4.24.44

Cloned(Commentary)

Cloned (Comment) Organism
-
 Crotalus atrox

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Crotalus atrox
EDTA
-
 Crotalus atrox
Human plasma alpha2-macroglobulin
-
 Crotalus atrox
additional information synthetic peptide inhibitors with N-[1-(R,S)-3-dicarboxypropyl] moiety Crotalus atrox
Pyroglutamate peptides e.g. pyro-Glu-Asn-Trp or pyro-Glu-Glu-Trp Crotalus atrox
Synthetic peptide inhibitors with N-[1-(R,S)-carboxypentyl] moiety
-
 Crotalus atrox

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can be directly exchanged for zinc by dialysis without loss of specificity or activity Crotalus atrox
Zn2+ zinc metalloproteinase Crotalus atrox
Zn2+ 1 mol zinc/mol enzyme Crotalus atrox

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25700
-
 1 * 25700, Crotalus atrox, SDS-PAGE Crotalus atrox

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Basement membranes surrounding capillaries + H2O Crotalus atrox contributes to hemorrhagic property by weakening capillary walls, together with atrolysins B and C member of the class P-I hemorrhagic toxins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Crotalus atrox
-
 Western diamondback rattlesnake
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information no glycoprotein Crotalus atrox

Purification (Commentary)

Purification (Comment) Organism
from lyophilized crude venom Crotalus atrox

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Crotalus atrox
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Basement membrane preparation + H2O
-
 Crotalus atrox Soluble peptides
-
 ?
Basement membranes surrounding capillaries + H2O contributes to hemorrhagic property by weakening capillary walls, together with atrolysins B and C member of the class P-I hemorrhagic toxins Crotalus atrox ?
-
 ?
Collagen type IV alphaI(IV) chains + H2O basement membrane component, cleavage at Ala219-Gln220 Crotalus atrox ?
-
 ?
Collagen type IV alphaII(IV) chain + H2O basement membrane component, cleavage at Thr228-Leu229 Crotalus atrox ?
-
 ?
Fibrinogen + H2O
-
 Crotalus atrox Hydrolyzed fibrinogen non-clotting products ?
Fibronectin + H2O
-
 Crotalus atrox Hydrolyzed fibronectin lower MW fragments ?
Gelatin of collagen type I + H2O
-
 Crotalus atrox ?
-
 ?
Gelatin of collagen type II + H2O
-
 Crotalus atrox ?
-
 ?
Gelatin of collagen type III + H2O
-
 Crotalus atrox ?
-
 ?
Gelatin of collagen type V + H2O
-
 Crotalus atrox ?
-
 ?
Human alpha2-macroglobulin + H2O cleavage at Val689-Met690 and Gly693-His694 Crotalus atrox ?
-
 ?
Laminin + H2O
-
 Crotalus atrox ?
-
 ?
Laminin/nidogen complex + H2O cleavage at positions 322, 336, 351, 840 and 953 Crotalus atrox ?
-
 ?
additional information no hydrolysis of fibrin Crotalus atrox ?
-
 ?
additional information no hydrolysis of native collagens Crotalus atrox ?
-
 ?
additional information substrate digestion patterns differ from those of atrolysins A and C Crotalus atrox ?
-
 ?
Nidogen + H2O basement membrane component, cleavage at positions 75, 296, 336, 402, 478, 625, 702 and 920 Crotalus atrox ?
-
 ?
Oxidized insulin B-chain + H2O
-
 Crotalus atrox Hydrolyzed oxidized insulin B-chain
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 25700, Crotalus atrox, SDS-PAGE Crotalus atrox

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 5.6 Crotalus atrox