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Literature summary for 3.4.24.42 extracted from

  • Mori, N.; Nikali, T.; Sugihara, H.; Tu, A.T.
    Biochemical characterization of hemorrhagic toxins with fibrinogenase activity isolated from Crotalus ruber ruber venom (1987), Arch. Biochem. Biophys., 253, 108-121.
    View publication on PubMed
Search for more literature for 3.4.24.42

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Crotalus ruber ruber
EDTA
-
 Crotalus ruber ruber
additional information benzamidine, tosyl-L-Phe chloromethyl ketone, soybean or egg white trypsin inhibitor or PCMB Crotalus ruber ruber

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.75 mol/mol toxin HT-3, 1.1 mol/mol toxin HT-2 Crotalus ruber ruber
additional information less than 0.3 mol Mg2+/mol toxin, less than 0.8 mol K+/mol toxin HT-3 and less than 0.9 mol K+/mol toxin HT-2 Crotalus ruber ruber
Na+ 8.5 mol/mol toxin HT-3, 10.3 mol/mol toxin HT-2 Crotalus ruber ruber
Zn2+ zinc metalloproteinase Crotalus ruber ruber
Zn2+ 1 mol zinc/mol enzyme Crotalus ruber ruber

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Crotalus ruber ruber
25000
-
 1 * 25000, Crotalus ruber ruber, toxin HT-2 Crotalus ruber ruber
25500
-
 1 * 25500, Crotalus ruber ruber, toxin HT-3 Crotalus ruber ruber

Organism

Organism UniProt Comment Textmining
Crotalus ruber ruber
-
 red rattlesnake
-

Purification (Commentary)

Purification (Comment) Organism
from lyophilized crude venom, toxins HT-2 and HT-3 Crotalus ruber ruber

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Crotalus ruber ruber
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu, cleaved at His-Pro and Pro-Phe Crotalus ruber ruber Asp-Arg-Val-Tyr-Ile-His + Pro + Phe-His-Leu
-
 ?
casein + H2O
-
 Crotalus ruber ruber ?
-
 ?
Dimethylcasein + H2O
-
 Crotalus ruber ruber ?
-
 ?
hide powder azure + H2O
-
 Crotalus ruber ruber ?
-
 ?
Luteinizing hormone-releasing hormone + H2O cleavage sites: Trp3-Ser4, Gly6-Leu7 Crotalus ruber ruber ?
-
 ?
Met-enkephalin + H2O i.e. Tyr-Gly-Gly-Phe-Met, cleavage site: Gly3-Phe4 Crotalus ruber ruber Tyr-Gly-Gly + Phe-Met
-
 ?
additional information hemorrhagic and myonecrotic activity Crotalus ruber ruber ?
-
 ?
Oxidized insulin B-chain + H2O cleavage at Gly23-Phe24 Crotalus ruber ruber ?
-
 ?
Oxidized insulin B-chain + H2O Crotalus ruber ruber toxin HT-3 does not cleave the His5-Leu6 bond which is cleaved by HT-2 Crotalus ruber ruber ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 25000, Crotalus ruber ruber, toxin HT-2 Crotalus ruber ruber
monomer 1 * 25500, Crotalus ruber ruber, toxin HT-3 Crotalus ruber ruber

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 inactivation Crotalus ruber ruber

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 5.2 (toxin HT-2), pI: 9.6 (toxin HT-3) Crotalus ruber ruber