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Literature summary for 3.4.24.40 extracted from

  • Merheb-Dini, C.; Cabral, H.; Leite, R.S.; Zanphorlin, L.M.; Okamoto, D.N.; Rodriguez, G.O.; Juliano, L.; Arantes, E.C.; Gomes, E.; da Silva, R.
    Biochemical and functional characterization of a metalloprotease from the thermophilic fungus Thermoascus aurantiacus (2009), J. Agric. Food Chem., 57, 9210-9217 .
    View publication on PubMed
Search for more literature for 3.4.24.40

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 mM, 90% residual activity Thermoascus aurantiacus
Cu2+ 10 mM, 58% residual activity Thermoascus aurantiacus
EDTA 10 mM, 3% residual activity Thermoascus aurantiacus
Hg2+ 10 mM, 5.5% residual activity Thermoascus aurantiacus
iodoacetamide 10 mM, 96% residual activity Thermoascus aurantiacus
Mg2+ 10 mM, 93% residual activity Thermoascus aurantiacus
phenylmethylsulfonyl fluoride 10 mM, 97% residual activity Thermoascus aurantiacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 2-aminobenzoyl-KLCSSKQ-N-(2,4-dinitrophenyl)ethylenediamine pH 5.5, 55°C Thermoascus aurantiacus
0.013
-
 2-aminobenzoyl-KLKSSKQ-N-(2,4-dinitrophenyl)ethylenediamine pH 5.5, 55°C Thermoascus aurantiacus
0.0173
-
 2-aminobenzoyl-KLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine pH 5.5, 55°C Thermoascus aurantiacus
0.024
-
 casein pH 5.5, 55°C Thermoascus aurantiacus
0.144
-
 azocasein pH 5.5, 55°C Thermoascus aurantiacus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermoascus aurantiacus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 10 mM, 197% of initial activity Thermoascus aurantiacus

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoascus aurantiacus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2310
-
 pH 5.5, 55°C Thermoascus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-KLCSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
-
 Thermoascus aurantiacus 2-aminobenzoyl-KLC + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
2-aminobenzoyl-KLKSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
-
 Thermoascus aurantiacus 2-aminobenzoyl-KLK + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
2-aminobenzoyl-KLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
-
 Thermoascus aurantiacus 2-aminobenzoyl-KLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
 ?
azocasein + H2O
-
 Thermoascus aurantiacus ?
-
 ?
casein + H2O
-
 Thermoascus aurantiacus ?
-
 ?
additional information enzyme prefers Arg in subsite P1 Thermoascus aurantiacus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 24500, SDS-PAGE Thermoascus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Thermoascus aurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 4 h, 70% residual activity Thermoascus aurantiacus
75
-
 2 h, 30% residual activity Thermoascus aurantiacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 2-aminobenzoyl-KLCSSKQ-N-(2,4-dinitrophenyl)ethylenediamine pH 5.5, 55°C Thermoascus aurantiacus
0.19
-
 2-aminobenzoyl-KLKSSKQ-N-(2,4-dinitrophenyl)ethylenediamine pH 5.5, 55°C Thermoascus aurantiacus
0.52
-
 2-aminobenzoyl-KLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine pH 5.5, 55°C Thermoascus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 substrates casein and azocasein Thermoascus aurantiacus
10.5
-
 substrate casein Thermoascus aurantiacus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5
-
 azocasein pH 5.5, 55°C Thermoascus aurantiacus
12.1
-
 casein pH 5.5, 55°C Thermoascus aurantiacus
12.7
-
 2-aminobenzoyl-KLCSSKQ-N-(2,4-dinitrophenyl)ethylenediamine pH 5.5, 55°C Thermoascus aurantiacus
14.9
-
 2-aminobenzoyl-KLKSSKQ-N-(2,4-dinitrophenyl)ethylenediamine pH 5.5, 55°C Thermoascus aurantiacus
30.1
-
 2-aminobenzoyl-KLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine pH 5.5, 55°C Thermoascus aurantiacus