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Literature summary for 3.4.24.40 extracted from

  • Ertan, H.; Cassel, C.; Verma, A.; Poljak, A.; Charlton, T.; Aldrich-Wright, J.; Omar, S.; Siddiqui, K.; Cavicchioli, R.
    A new broad specificity alkaline metalloprotease from a Pseudomonas sp. isolated from refrigerated milk: Role of calcium in improving enzyme productivity (2015), J. Mol. Catal. B, 113, 1-8.
No PubMed abstract available
Search for more literature for 3.4.24.40

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ leads to an increase in protease productivity by 300% Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. RC
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, anion exchange column chromatography, and gel filtration Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Pseudomonas sp. ?
-
 ?
azocasein + H2O
-
 Pseudomonas sp. RC ?
-
 ?
Bovine serum albumin + H2O the enzyme cleaves 61 unique sequence pairs of bovine serum albumin involving all 20 amino acids Pseudomonas sp. ?
-
 ?
Bovine serum albumin + H2O the enzyme cleaves 61 unique sequence pairs of bovine serum albumin involving all 20 amino acids Pseudomonas sp. RC ?
-
 ?

Synonyms

Synonyms Comment Organism
AMP-P
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42 48 the enzyme shows a half-life of 4.9 min, 3.4 min, 2.2 min, and 1.2 min at 42°C, 44°C, 46°C, and 48°C, respectively Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.045
-
 azocasein at pH 6.5 and 25°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.2 8.8 more than 90% of maximal activity between pH 6.0 and 8.8, 44% activity at pH 5.2 Pseudomonas sp.