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Literature summary for 3.4.24.40 extracted from

  • Wang, F.; Hao, J.; Yang, C.; Sun, M.
    Cloning, Expression, and Identification of a Novel Extracellular Cold-Adapted Alkaline Protease Gene of the Marine Bacterium Strain YS-80-122 (2010), Appl. Biochem. Biotechnol., 162, 1497-1505.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinantly expressed in Escherichia coli Flavobacterium sp. YS-80-122

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
SDS-PAGE Flavobacterium sp. YS-80-122

Organism

Organism UniProt Comment Textmining
Flavobacterium sp. YS-80-122 D0VMS8
-
-

Purification (Commentary)

Purification (Comment) Organism
purified to homogeneity by precipitation with ammonium sulfate (65%). After dialysis, the crude enzyme is further purified by FPLC using Superdex 200 column Flavobacterium sp. YS-80-122

Synonyms

Synonyms Comment Organism
LupA sequence alignment reveals that the protein belongs to the serralysin-type metalloproteases Flavobacterium sp. YS-80-122