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Literature summary for 3.4.24.40 extracted from

  • Dufour, D.; Nicodeme, M.; Perrin, C.; Driou, A.; Brusseaux, E.; Humbert, G.; Gaillard, J.L.; Dary, A.
    Molecular typing of industrial strains of Pseudomonas spp. isolated from milk and genetical and biochemical characterization of an extracellular protease produced by one of them (2008), Int. J. Food Microbiol., 125, 188-196.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 5 mM, 5% residual activity Pseudomonas fluorescens
EDTA 5 mM, 23% residual activity Pseudomonas fluorescens
EGTA 5 mM, 33% residual activity Pseudomonas fluorescens
NaCl 1 M, 56% residual activity Pseudomonas fluorescens
o-phenanthroline 0.5 mM, 10% residual activity Pseudomonas fluorescens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Pseudomonas fluorescens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens Q3YAW3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas fluorescens

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Pseudomonas fluorescens
More the N-terminal amino acid sequence shares 100% identity with the domain 14-34 of extracellular alkaline endoprotease sequences called AprX Pseudomonas fluorescens

Synonyms

Synonyms Comment Organism
AprX
-
Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Pseudomonas fluorescens

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas fluorescens isoelectric focusing
-
4