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Literature summary for 3.4.24.40 extracted from

  • Walasek, P.; Honek, J.F.
    Nonnatural amino acid incorporation into the methionine 214 position of the metzincin Pseudomonas aeruginosa alkaline protease (2005), BMC Biochem., 6, 21.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.40

Protein Variants

Protein Variants Comment Organism
additional information recombinant protein labeled with L-difluoromethionine in invariant position of M214 shows little effect on protein structure or function Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 benzyloxycarbonyl-L-Arg-L-Arg-4-nitroanilide recombinant enzyme, pH 7.8 Pseudomonas aeruginosa
0.0126
-
 benzyloxycarbonyl-L-Arg-L-Arg-4-nitroanilide native enzyme, pH 7.8 Pseudomonas aeruginosa
0.014
-
 benzyloxycarbonyl-L-Arg-L-Arg-4-nitroanilide recombinant enzyme labeled with L-difluoromethionine, pH 7.8 Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49500
-
 x * 49500, full length mature enzyme, mass spectrometry Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 native and recombinant protein
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-L-Arg-L-Arg-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?

Subunits

Subunits Comment Organism
? x * 49500, full length mature enzyme, mass spectrometry Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
AprA
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0073
-
 benzyloxycarbonyl-L-Arg-L-Arg-4-nitroanilide recombinant enzyme labeled with L-difluoromethionine, pH 7.8 Pseudomonas aeruginosa
0.01
-
 benzyloxycarbonyl-L-Arg-L-Arg-4-nitroanilide recombinant enzyme, pH 7.8 Pseudomonas aeruginosa
0.026
-
 benzyloxycarbonyl-L-Arg-L-Arg-4-nitroanilide native enzyme, pH 7.8 Pseudomonas aeruginosa