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Literature summary for 3.4.24.40 extracted from

  • Kim, H.J.; Tamanoue, Y.; Jeohn, G.H.; Iwamatsu, A.; Yokota, A.; Kim, Y.T.; Takahashi, T.; Takahashi, K.
    Purification and characterization of an extracellular metalloprotease from Pseudomonas fluorescens (1997), J. Biochem., 121, 82-88.
    View publication on PubMed
Search for more literature for 3.4.24.40

Activating Compound

Activating Compound Comment Organism Structure
t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide
-
 Pseudomonas fluorescens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 10 mM, strong inhibition at pH 5.5 and pH 8.0 with and without addition of 1 mM Co2+ Pseudomonas fluorescens
Bovine pancreatic trypsin inhibitor 0.01 mM, 5% inhibition at pH 5.5 Pseudomonas fluorescens
chymostatin 0.1 mM, 18% inhibition at pH 8.0 Pseudomonas fluorescens
diisopropylfluorophosphate 1 mM, weak inhibition at pH 5.5 and pH 8.0 with and without addition of 1 mM Co2+ Pseudomonas fluorescens
EDTA 10 mM, almost complete inactivation at pH 5.5 and pH 8.0 with and without addition of 1 mM Co2+ Pseudomonas fluorescens
Elastatinal 0.1 mM, 11% inhibition at pH 8.0 and without 1 mM Co2+ addition Pseudomonas fluorescens
L-trans-epoxysuccinyl-leucyl-amido-(4-guanido)butane 0.1 mM, 26% inhibition at pH 8.0 and 1 mM Co2+ addition, 26% inhibition at pH 5.5 without addition of 1 mM Co2+ Pseudomonas fluorescens
leupeptin 0.1 mM, 10% inhibition at pH 8.0 and 1 mM Co2+ addition, 7% inhibition at pH 8.0 without 1 mM Co2+ addition Pseudomonas fluorescens
N-ethylmaleimide 0.1 mM, 14% inhibition at pH 8.0 and 1 mM Co2+ addition Pseudomonas fluorescens
N-tosyl-L-phenylalanine chloromethyl ketone 0.1 mM, 18% inhibition at pH 8.0 Pseudomonas fluorescens
phenyl-methanesulfonyl fluoride 1 mM, 14-17% inhibition at pH 5.5 and pH 8.0 with and without addition of 1 mM Co2+ Pseudomonas fluorescens
phosphoramidon 0.1 mM, no inhibition at pH 8.0 and 1 mM Co2+ addition, 25% inhibition at pH 8.0 and 5.5 without addition of 1 mM Co2+ Pseudomonas fluorescens
Soybean trypsin inhibitor 0.01 mM, 9% inhibition at pH 5.5 Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide without Co2+, pH 8.0 Pseudomonas fluorescens
0.016
-
 t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide without Co2+, pH 8.0 Pseudomonas fluorescens
0.023
-
 t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide 1 mM Co2+, pH 5.5 Pseudomonas fluorescens
0.023
-
 t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide 1 mM Co2+, pH 8.0 Pseudomonas fluorescens
0.023
-
 t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide without Co2+, pH 5.5 Pseudomonas fluorescens
0.035
-
 t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide 1 mM Co2+, pH 8.0 Pseudomonas fluorescens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas fluorescens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 gel filtration Pseudomonas fluorescens
47000
-
 SDS-PAGE Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
 KT1
-
Pseudomonas fluorescens KT1
-
 KT1
-

Purification (Commentary)

Purification (Comment) Organism
dialysis against 10 mM N-methyldiethanolamine, DE-52 gel filtration, chelating Sepharose 6B gel filtration, dialysis against 0.02M triethanolamine-HCl buffer Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.98
-
 7-amino-4-methyl-coumarin, 37°C, pH 8.0 Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
t-butyloxycarbonyl-Ala-Gly-Pro-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
t-butyloxycarbonyl-Ala-Gly-Pro-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens KT1 ?
-
 ?
t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens KT1 ?
-
 ?
t-butyloxycarbonyl-Gln-Ala-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
t-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens KT1 ?
-
 ?
t-butyloxycarbonyl-Glu-Lys-Lys-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
t-butyloxycarbonyl-Gly-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
t-butyloxycarbonyl-Gly-Lys-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
t-butyloxycarbonyl-Leu-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
t-butyloxycarbonyl-Leu-Lys-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
t-butyloxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
t-butyloxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens KT1 ?
-
 ?
t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens ?
-
 ?
t-butyloxycarbonyl-Val-Leu-Lys-4-methylcoumaryl-7-amide + H2O
-
 Pseudomonas fluorescens KT1 ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 with substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide in absence and presence of Co2+ Pseudomonas fluorescens

pH Range

pH Minimum pH Maximum Comment Organism
6 8 in absence and presence of Co2+ Pseudomonas fluorescens
8 9 with substrate t-butyloxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumaryl-7-amide in the presence of Co2+ Pseudomonas fluorescens