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Literature summary for 3.4.24.40 extracted from
- Metal ion relevance for the stability and activity of a 52-kDa Serratia proteinase (1993), Biosci. Biotechnol. Biochem., 57, 29-33.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | Ca2+ (restored activity higher than native activity); enhances inhibition by guanidine-HCl; Fe2+ (partially); Mg2+; Mn2+ (restored activity higher than native activity); no reactivation by K+; Zn2+ (restored activity higher than native activity) | Serratia marcescens |  |
| Guanidine-HCl | enhanced by EDTA | Serratia marcescens | |
| additional information | PCMB | Serratia marcescens | |
| PMSF | not | Serratia marcescens | |
| Tetraethylenepentamine | zinc-specific chelator | Serratia marcescens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Serratia marcescens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc-containing metalloproteinase | Serratia marcescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
1 * 52000, Serratia marcescens, SDS-PAGE | Serratia marcescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Serratia marcescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Serratia marcescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Azocoll + H2O | - |
Serratia marcescens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 52000, Serratia marcescens, SDS-PAGE | Serratia marcescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal unfolding profiles | Serratia marcescens |
| 55 | - |
the selective substitution of Ca2+ for functional zinc does not change stability below 55°¦C | Serratia marcescens |
| 60 | - |
inactivation in 20 mM Tris-HCl buffer, pH 7, stable in the presence of tetraethylenepentamine or CaCl2 | Serratia marcescens |
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