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Literature summary for 3.4.24.40 extracted from
- Pseudomonas aeruginosa proteinase. I. Purification and general properties (1963), Biochim. Biophys. Acta, 73, 113-124.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| Pseudomonas aeruginosa | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 10 mM; strong | Pseudomonas aeruginosa |  |
| 2,2'-bipyridyl | not | Pseudomonas aeruginosa | |
| Cu2+ | - |
Pseudomonas aeruginosa | |
| EDTA | Co2+; Mn2+; strong, 5 mM | Pseudomonas aeruginosa | |
| Fe2+ | strong | Pseudomonas aeruginosa | |
| Hg2+ | - |
Pseudomonas aeruginosa | |
| KCN | not | Pseudomonas aeruginosa | |
| KMnO4 | - |
Pseudomonas aeruginosa | |
| additional information | citrate, NaF, oxalate; diisopropyl phosphofluoridate; monoiodoacetic acid; PCMB; potato inhibitor; soybean trypsin inhibitor | Pseudomonas aeruginosa | |
| N-bromosuccinimide | - |
Pseudomonas aeruginosa | |
| Pb2+ | - |
Pseudomonas aeruginosa | |
| Sodium thioglycolate | not | Pseudomonas aeruginosa | |
| Urea | - |
Pseudomonas aeruginosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Pseudomonas aeruginosa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Egg albumin + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Gelatin + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Hemoglobin + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| additional information | benzoyl-L-Arg amide, acetyl-L-Tyr ethyl ester, carbobenzoxy-Glu-Tyr, carbobenzoxy-Gly-Phe, L-Leu amide | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | collagen | Pseudomonas aeruginosa | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Pseudomonas aeruginosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | 62 | about half-maximal activity at 42°C and 62°C | Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
pH 5-9, fairly stable | Pseudomonas aeruginosa |
| 50 | - |
below, 10 min, fairly stable at neutral pH-values | Pseudomonas aeruginosa |
| 55 | - |
10 min, partly unstable in distilled water, 100 mM phosphate or 10 mM CaCl2 stabilizes | Pseudomonas aeruginosa |
| 63 | - |
10 min, inactivation in the presence of stabilizing agents | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 4 | Pseudomonas aeruginosa |
| 7 | 9 | egg white albumin, hemoglobin as substrates | Pseudomonas aeruginosa |
| 8 | 9 | casein as substrate | Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | about half-maximal activity at pH 6 and about 80% of maximal activity at pH 10, casein as substrate, about 75% of maximal activity at pH 6 and about 60% of maximal activity at pH 10, egg white albumin as substrate | Pseudomonas aeruginosa |
| 6.5 | 10 | about half-maximal activity at pH 6.5 and about 65% of maximal activity at pH 10, hemoglobin as substrate | Pseudomonas aeruginosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 11 | stable in this range | Pseudomonas aeruginosa |
| 5 | 9 | 10 min, fairly stable at 30°C | Pseudomonas aeruginosa |
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