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Literature summary for 3.4.24.40 extracted from
- Purification and characterization of the extracellular proteinase of Serratia marcescens (1979), Biochim. Biophys. Acta, 569, 293-301.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | neither Ca2+, Zn2+ nor Fe2+ restores | Serratia marcescens |  |
| EDTA | Ca2+ (not) restores; Fe2+ (not); Zn2+ (not) | Serratia marcescens | |
| additional information | - |
Serratia marcescens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Serratia marcescens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Serratia marcescens |
| 47500 | - |
1 * 47500, Serratia marcescens, SDS-PAGE | Serratia marcescens |
| 51900 | - |
Serratia marcescens, sedimentation equilibrium centrifugation | Serratia marcescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Serratia marcescens | - |
derepressed strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to near homogeneity | Serratia marcescens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Serratia marcescens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Serratia marcescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, lyophilized Serratia marcescens enzyme, several months | Serratia marcescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Aminoethylated lysozyme + H2O | - |
Serratia marcescens | ? | - |
? | |
| C-terminal octapeptide of glucagon + H2O | cleavage sites | Serratia marcescens | ? | - |
? | |
| Carboxymethyl-beta-insulin + H2O | cleavage sites | Serratia marcescens | ? | - |
? | |
| casein + H2O | - |
Serratia marcescens | ? | - |
? | |
| Glucagon + H2O | cleavage sites | Serratia marcescens | ? | - |
? | |
| Hemoglobin + H2O | - |
Serratia marcescens | ? | - |
? | |
| additional information | no substrates are small peptides | Serratia marcescens | ? | - |
? | |
| additional information | minimum peptide size: hexapeptide | Serratia marcescens | ? | - |
? | |
| additional information | no esterase activity: no substrates are N-benzoyl-L-Arg ethyl ester or N-benzoyl-L-Tyr ethyl esters | Serratia marcescens | ? | - |
? | |
| N-terminal hexapeptide of glucagon + H2O | cleavage sites | Serratia marcescens | ? | - |
? | |
| Ribonuclease + H2O | - |
Serratia marcescens | ? | - |
? | |
| Tryptic insulin heptapeptide + H2O | cleavage sites | Serratia marcescens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47500, Serratia marcescens, SDS-PAGE | Serratia marcescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 10.6 | - |
Serratia marcescens |
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