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Literature summary for 3.4.24.40 extracted from

  • Lyerly, D.; Kreger, A.
    Purification and characterization of a Serratia marcescens metalloprotease (1979), Infect. Immun., 24, 411-421.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.40

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline reversible by dialysis, Mg2+ or Co2+ partially restores; strong Serratia marcescens
2,2'-bipyridyl weak Serratia marcescens
Ag2+ strong Serratia marcescens
Co2+ weak Serratia marcescens
Cu2+
-
 Serratia marcescens
Cysteine hydrochloride weak Serratia marcescens
DTT
-
 Serratia marcescens
EDTA Ca2+ (partially); Cu2+ restores partially; more effective at acidic pH; partially reversible by dialysis Serratia marcescens
Fe2+
-
 Serratia marcescens
Hg2+
-
 Serratia marcescens
KMnO4
-
 Serratia marcescens
additional information Lima bean, human pancreatic or egg white trypsin inhibitor, human alpha1-antitrypsin; no inhibition by Mg2+, Mn2+, Ca2+, Ba2+, NaCN, NaN3, sodium arsenite, p-hydroxymercuribenzoate; soybean trypsin inhibitor Serratia marcescens
N-bromosuccinimide strong Serratia marcescens
N-ethylmaleimide weak Serratia marcescens
nitrilotriacetate weak Serratia marcescens
Pb2+
-
 Serratia marcescens
PMSF not Serratia marcescens
Sodium thioglycolate weak Serratia marcescens
Zn2+ strong Serratia marcescens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Serratia marcescens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ requirement Serratia marcescens
Ca2+ 7 gatom/mol enzyme Serratia marcescens
Co2+ not Serratia marcescens
Mg2+ not Serratia marcescens
additional information no activation by Fe3+, Mg2+ Serratia marcescens
additional information no activation by Mn2+ Serratia marcescens
Zn2+ added Zn2+ inhibits Serratia marcescens
Zn2+ zinc-containing metalloproteinase Serratia marcescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Serratia marcescens
44000
-
 Serratia marcescens, gel filtration Serratia marcescens
52500
-
 1 * 52500, Serratia marcescens, SDS-PAGE Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
-
-
Serratia marcescens BG
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Serratia marcescens

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Serratia marcescens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Serratia marcescens

Storage Stability

Storage Stability Organism
4°C, 5 mg enzyme/ml deionized glass-distilled water, 1 day Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Serratia marcescens ?
-
 ?
azocasein + H2O
-
 Serratia marcescens BG ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 52500, Serratia marcescens, SDS-PAGE Serratia marcescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 1 h, pH 5-10 Serratia marcescens
25 45 15 min, stable at pH 7.5 Serratia marcescens
60
-
 15 min, inactivation Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 5.3-5.4 Serratia marcescens
5.5 7.5 azocasein as substrate Serratia marcescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 4 inactivation Serratia marcescens
5 10 1 h, at room temperature Serratia marcescens
7.5
-
 15 min, stable at 25-45°C Serratia marcescens