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Literature summary for 3.4.24.40 extracted from
- Purification and properties of an extracellular proteinase of psychrophilic Escherichia freundii (1974), Eur. J. Biochem., 44, 87-96.
General Stability
| General Stability | Organism |
|---|---|
| Unstable in the presence of 10 mM CaCl2 | Citrobacter freundii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1 mM | Citrobacter freundii |  |
| 2,2'-bipyridyl | 5 mM | Citrobacter freundii | |
| 8-hydroxyquinoline | 0.1 mM at pH 10, 5 mM at pH 7 | Citrobacter freundii | |
| benzalkonium chloride | - |
Citrobacter freundii | |
| Cd2+ | at pH 10, not at pH 7 | Citrobacter freundii | |
| EDTA | 0.1 mM at pH 10, 5 mM at pH 7 | Citrobacter freundii | |
| Hg2+ | - |
Citrobacter freundii | |
| KCN | at pH 10, no inhibition at pH 7.5 | Citrobacter freundii | |
| additional information | 2-mercaptoethanol; diisopropyl phosphofluoridate; iodine, ovomucoid; monoiodoacetic acid; PCMB; potato inhibitor; soybean trypsin inhibitor | Citrobacter freundii | |
| N-bromosuccinimide | strong | Citrobacter freundii | |
| Ni2+ | at pH 10, not at pH 7 | Citrobacter freundii | |
| Sodium thioglycolate | 1 mM, at pH 10, no inhibition at pH 7.5 | Citrobacter freundii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Citrobacter freundii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | requirement | Citrobacter freundii | |
| Co2+ | activation | Citrobacter freundii | |
| Co2+ | 1 mM, at pH 10, not at pH 7 | Citrobacter freundii | |
| additional information | atomic absorption spectroscopy | Citrobacter freundii | |
| additional information | magnesium, manganese, cobalt, copper or iron | Citrobacter freundii | |
| Zn2+ | zinc-containing metalloproteinase | Citrobacter freundii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Citrobacter freundii |
| 45000 | - |
Escherichia freundii, gel filtration | Citrobacter freundii |
| 45400 | - |
Escherichia freundii, minimum MW calculated on the basis of zinc content | Citrobacter freundii |
| 45800 | - |
Escherichia freundii, sedimentation equilibrium studies | Citrobacter freundii |
| 51000 | - |
1 * 51000, Escherichia freundii, SDS-PAGE | Citrobacter freundii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | - |
psychrophilic strain B-3038 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 0.69 mol sugar/mol enzyme | Citrobacter freundii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Citrobacter freundii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Citrobacter freundii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2174 | - |
- |
Citrobacter freundii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, pH 4.5-11.5, 1 day | Citrobacter freundii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Citrobacter freundii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 51000, Escherichia freundii, SDS-PAGE | Citrobacter freundii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
at pH 10, in sodium carbonate buffer | Citrobacter freundii |
| 40 | - |
at pH 8, in phosphate buffer | Citrobacter freundii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
3 h, stable at pH 5-10 | Citrobacter freundii |
| 25 | - |
and below, 10 min stable at pH 10 | Citrobacter freundii |
| 30 | - |
1 h, stable at pH 6-8.5 | Citrobacter freundii |
| 35 | - |
10 min, pH 10, inactivation | Citrobacter freundii |
| 45 | - |
and below, 10 min stable at pH 6.5 | Citrobacter freundii |
| 55 | - |
10 min, inactivation, CaCl2 does not stabilize | Citrobacter freundii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.2 | Citrobacter freundii |
| 10 | - |
- |
Citrobacter freundii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 10.8 | about half-maximal activity at pH 7.2 and 10.8 | Citrobacter freundii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | 3 h, stable at 30°C | Citrobacter freundii |
| 6 | 8.5 | 1 h, stable at 30°C | Citrobacter freundii |
| 6.5 | - |
10 min, stable below 45°C | Citrobacter freundii |
| 10 | - |
10 min, stable below 25°C, inactivation at 35°C | Citrobacter freundii |
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