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Literature summary for 3.4.24.40 extracted from
- On the specificity of Pseudomonas aeruginosa alkaline proteinase with synthetic peptides (1973), Biochim. Biophys. Acta, 309, 414-429.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| benzyloxycarbonyl-D-Ala-Leu-Ala | competitive to benzyloxycarbonyl-Ala-Phe-Gly-Ala | Pseudomonas aeruginosa |  |
| Benzyloxycarbonyl-Gly-Leu-D-Ala | benzyloxycarbonyl-Ala-Phe-Gly-Ala as substrate | Pseudomonas aeruginosa | |
| Benzyloxycarbonyl-Gly-Leu-Gly | benzyloxycarbonyl-Ala-Phe-Gly-Ala as substrate | Pseudomonas aeruginosa | |
| Benzyloxycarbonyl-Gly-Leu-NH2 | benzyloxycarbonyl-Ala-Phe-Gly-Ala as substrate | Pseudomonas aeruginosa | |
| Benzyloxycarbonyl-Phe-D-Leu-Ala | competitive to benzyloxycarbonyl-Ala-Phe-Gly-Ala | Pseudomonas aeruginosa | |
| additional information | diisopropyl phosphofluoridate; PCMB; potato inhibitor; soybean trypsin inhibitor; tosyl-L-Phe chloromethyl ketone or tosyl-L-Lys chloromethyl ketone | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
benzyloxycarbonyl-Phe-Phe-Ala | - |
Pseudomonas aeruginosa | |
| 0.4 | - |
Benzyloxycarbonyl-Phe-Ala-Ala | benzyloxycarbonyl-Phe-Leu-Ala | Pseudomonas aeruginosa | |
| 0.5 | - |
Ala-Ala-Ala-Ala-Ala-Ala | benzyloxycarbonyl-Phe-L-Ala-Ala | Pseudomonas aeruginosa | |
| 0.6 | - |
benzyloxycarbonyl-Gly-Leu-Phe | benzyloxycarbonyl-Ala-Phe-Leu-Ala | Pseudomonas aeruginosa | |
| 0.9 | - |
Benzyloxycarbonyl-Gly-Leu-Leu | benzyloxycarbonyl-Gly-Phe-Leu-Ala | Pseudomonas aeruginosa | |
| 1.2 | - |
Ala-Ala-Ala-Ala-Ala | - |
Pseudomonas aeruginosa | |
| 1.5 | - |
benzyloxycarbonyl-Ala-Leu-Ala | - |
Pseudomonas aeruginosa | |
| 1.8 | - |
benzyloxycarbonyl-Phe-Gly-Ala | benzyloxycarbonyl-Phe-Ser-Ala, benzyloxycarbonyl-Leu-Ala-Ala | Pseudomonas aeruginosa | |
| 2.3 | - |
benzyloxycarbonyl-Phe-Gly-Leu-Ala | benzyloxycarbonyl-Ala-Leu-Gly-Gly | Pseudomonas aeruginosa | |
| 2.4 | - |
benzyloxycarbonyl-Gly-Leu-Gly-Ala | - |
Pseudomonas aeruginosa | |
| 2.5 | - |
benzyloxycarbonyl-Val-Ala-Ala | benzyloxycarbonyl-Ala-Phe-Gly-Ala | Pseudomonas aeruginosa | |
| 2.7 | - |
Benzyloxycarbonyl-Ala-Ala-Ala | - |
Pseudomonas aeruginosa | |
| 2.9 | - |
Benzyloxycarbonyl-Gly-Leu-Ala | - |
Pseudomonas aeruginosa | |
| 4.3 | - |
Benzyloxycarbonyl-Gly-Leu-Gly-Gly-Ala | - |
Pseudomonas aeruginosa | |
| 4.8 | - |
benzyloxycarbonyl-Ala-Gly-Leu-Ala | - |
Pseudomonas aeruginosa | |
| 5.3 | - |
Benzyloxycarbonyl-Gly-Pro-Gly-Gly-Pro-Ala | - |
Pseudomonas aeruginosa | |
| 5.4 | - |
benzyloxycarbonyl-Gly-Gly-Leu-Ala | benzyloxycarbonyl-Ala-Gly-Gly-Leu | Pseudomonas aeruginosa | |
| 7.7 | - |
Benzyloxycarbonyl-Gly-Ala-Ala | - |
Pseudomonas aeruginosa | |
| 8.7 | - |
Ala-Ala-Ala-Ala | - |
Pseudomonas aeruginosa | |
| 11 | - |
Benzyloxycarbonyl-Gly-Leu-Gly-Gly | - |
Pseudomonas aeruginosa | |
| 17.3 | - |
Phe-Gly-Leu-Ala | - |
Pseudomonas aeruginosa | |
| 20 | - |
benzyloxycarbonyl-D-Ala-Gly-Leu-Ala | - |
Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | no activation by added Ca2+ | Pseudomonas aeruginosa | |
| Ca2+ | 1-2 gatom/mol enzyme | Pseudomonas aeruginosa | |
| Co2+ | activation | Pseudomonas aeruginosa | |
| Co2+ | above 5 mM, benzoxycarbonyl-Ala-Phe-Gly-Ala as substrate | Pseudomonas aeruginosa | |
| additional information | contains no significant amount of any metal except Ca2+ | Pseudomonas aeruginosa | |
| additional information | no activation by Mn2+ | Pseudomonas aeruginosa | |
| additional information | no activation by Ni2+ | Pseudomonas aeruginosa | |
| Zn2+ | zinc-containing metalloproteinase (not) | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48400 | - |
Pseudomonas aeruginosa, sedimentation velocity centrifugation | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzyloxycarbonyl tripeptides + H2O | overview | Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Ala-Ala-Ala + H2O | cleavage site: AlaÄ+ÄAla-Ala | Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Ala-Gly-Gly-Leu-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Ala-Gly-Gly-Leu-Xaa + H2O | a hydrophobic or bulky residue at P3' results in marked increase in hydrolysis | Pseudomonas aeruginosa | ? | - |
? | |
| benzyloxycarbonyl-Ala-Gly-Leu-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| benzyloxycarbonyl-Ala-Leu-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| benzyloxycarbonyl-D-Ala-Gly-Leu-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Ala-Ala + H2O | cleavage site: Gly-Ala | Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Gly-Gly-Leu + H2O | poor substrate | Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Gly-Leu amide + H2O | poor substrate | Pseudomonas aeruginosa | ? | - |
? | |
| benzyloxycarbonyl-Gly-Gly-Leu-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Leu-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| benzyloxycarbonyl-Gly-Leu-Gly-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Leu-Gly-Gly + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Leu-Gly-Gly-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Leu-Gly-Gly-Xaa + H2O | a hydrophobic or bulky residue at P3' results in marked increase in hydrolysis | Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Leu-Leu + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Leu-Phe + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Leu-Xaa + H2O | at position Xaa with decreasing susceptibility to the enzyme: Leu, Phe or Ala, poor substrates: Gly or NH2, D-Ala | Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Gly-Pro-Gly-Gly-Pro-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Phe-Ala-Ala + H2O | cleavage site: Phe-Ala | Pseudomonas aeruginosa | ? | - |
? | |
| benzyloxycarbonyl-Phe-Gly-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| benzyloxycarbonyl-Phe-Gly-Leu-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Phe-Leu-Ala-Ala + H2O | best substrate | Pseudomonas aeruginosa | ? | - |
? | |
| benzyloxycarbonyl-Phe-Phe-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Phe-Xaa-Ala + H2O | susceptibility to the enzyme is Xaa-dependent, in decreasing order of efficiency: Ala, Phe, Leu, Trp or Ser or Gly | Pseudomonas aeruginosa | Benzyloxycarbonyl-Phe + Xaa-Ala | - |
? | |
| benzyloxycarbonyl-Val-Ala-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Xaa-Ala-Ala + H2O | susceptibility to the enzyme is Xaa-dependent, in decreasing order of efficiency: L-Phe, Leu or Ala, Val, Gly, D-Ala | Pseudomonas aeruginosa | Benzyloxycarbonyl-Xaa + Ala-Ala | - |
? | |
| Benzyloxycarbonyl-Xaa-Gly-Leu-Ala + H2O | a hydrophobic or bulky residue at P2 results in marked increase in hydrolysis | Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Xaa-Leu-Ala + H2O | susceptibility to the enzyme is Xaa-dependent, in decreasing order of efficiency: L-Phe, Leu or Ala, Val, Gly, D-Ala | Pseudomonas aeruginosa | Benzyloxycarbonyl-Xaa + Leu-Ala | - |
? | |
| Benzyloxycarbonyl-Xaa-Phe-Gly-Ala + H2O | a hydrophobic or bulky residue at P2 results in marked increase in hydrolysis | Pseudomonas aeruginosa | ? | - |
? | |
| Benzyloxycarbonyl-Xaa-Phe-Leu-Ala + H2O | a hydrophobic or bulky residue at P2 results in marked increase in hydrolysis | Pseudomonas aeruginosa | ? | - |
? | |
| L-Ala-L-Ala-L-Ala-L-Ala + H2O | - |
Pseudomonas aeruginosa | L-Ala-L-Ala + L-Ala-L-Ala | - |
? | |
| L-Ala-L-Ala-L-Ala-L-Ala-L-Ala + H2O | - |
Pseudomonas aeruginosa | L-Ala-L-Ala + L-Ala-L-Ala-L-Ala | - |
? | |
| L-Ala-L-Ala-L-Ala-L-Ala-L-Ala-L-Ala + H2O | - |
Pseudomonas aeruginosa | L-Ala-L-Ala-L-Ala + L-Ala-L-Ala-L-Ala | - |
? | |
| L-Ala-oligopeptides + H2O | proteolytic activity increases drastically with increasing chain length from tetramer to hexamer, no substrate: dipeptide or tripeptide | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | elastin | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | tetrapeptides are poorer substrates than benzyloxycarbonyl tetrapeptides | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | benzyloxycarbonyl dipeptides, N-acetylated amino acid esters or amides, dipeptide amides, tripeptides, benzyloxycarbonyl-(Gly)4, benzyloxycarbonyl-(Gly)5, benzyloxycarbonyl-Gly-Leu-Gly-Gly-D-Ala | Pseudomonas aeruginosa | ? | - |
? | |
| Oxidized insulin B-chain + H2O | cleavage sites | Pseudomonas aeruginosa | ? | - |
? | |
| Phe-Gly-Leu-Ala + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 4 | Pseudomonas aeruginosa |
| 7 | - |
benzyloxycarbonyl-Phe-Ala-Ala or benzyloxycarbonyl-Ala-Phe-Gly-Ala as substrate | Pseudomonas aeruginosa |
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