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Literature summary for 3.4.24.39 extracted from

  • Fushimi, N.; Ee, C.E.; Nakajima, T.; Ichishima, E.
    Aspzincin, a family of metalloendopeptidases with a new zinc-binding motif. Identification of new zinc-binding sites (His(128), His(132), and Asp(164)) and three catalytically crucial residues (Glu(129), Asp(143), and Tyr(106)) of deuterolysin from Aspergillus oryzae by site-directed mutagenesis (1999), J. Biol. Chem., 274, 24195-24201.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme expressed in Escherichia coli Aspergillus oryzae

Protein Variants

Protein Variants Comment Organism
D104N site-directed mutagenesis Aspergillus oryzae
D121N site-directed mutagenesis Aspergillus oryzae
D143E site-directed mutagenesis Aspergillus oryzae
D143N site-directed mutagenesis Aspergillus oryzae
D164N site-directed mutagenesis Aspergillus oryzae
D80N site-directed mutagenesis Aspergillus oryzae
E129D site-directed mutagenesis Aspergillus oryzae
E129Q site-directed mutagenesis Aspergillus oryzae
E142Q site-directed mutagenesis Aspergillus oryzae
E42Q site-directed mutagenesis Aspergillus oryzae
E65Q site-directed mutagenesis Aspergillus oryzae
E86Q site-directed mutagenesis Aspergillus oryzae
H118A site-directed mutagenesis Aspergillus oryzae
H128R site-directed mutagenesis Aspergillus oryzae
H132R site-directed mutagenesis Aspergillus oryzae
R58Q site-directed mutagenesis Aspergillus oryzae
Y106F site-directed mutagenesis Aspergillus oryzae

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains 1 g-atom of zinc/mol of enzyme Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19020
-
 amino acid sequence Aspergillus oryzae
19800
-
 recombinant enzyme, gel filtration Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
 mold
-

Purification (Commentary)

Purification (Comment) Organism
recombinant prodeuterolysin Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
salmon protamine sulfate + H2O
-
 Aspergillus oryzae ?
-
 ?

Synonyms

Synonyms Comment Organism
aspzincin
-
 Aspergillus oryzae
Neutral proteinase II fornerly designated as Aspergillus oryzae