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Literature summary for 3.4.24.38 extracted from
- Gametolysin (2004), Handbook of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 592-595.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | degrades gametic cell walls, cell walls of vegetative cells and those of mother sporangial cells | Chlamydomonas reinhardtii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Chlamydomonas reinhardtii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Chlamydomonas reinhardtii |  |
| Alpha-macroglobulin | - |
Chlamydomonas reinhardtii | |
| EDTA | - |
Chlamydomonas reinhardtii | |
| EGTA | - |
Chlamydomonas reinhardtii | |
| HgCl2 | - |
Chlamydomonas reinhardtii | |
| p-chloromercuribenzoic acid | - |
Chlamydomonas reinhardtii | |
| phosphoramidon | inhibits at a high concentration | Chlamydomonas reinhardtii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | proenzyme | Chlamydomonas reinhardtii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slightly stimulates at 0.75-1 mM | Chlamydomonas reinhardtii | |
| Mg2+ | slightly stimulates at 0.75-1 mM | Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49630 | - |
mature enzyme | Chlamydomonas reinhardtii |
| 60000 | 62000 | active enzyme secreted into the mating medium | Chlamydomonas reinhardtii |
| 65000 | - |
proenzyme | Chlamydomonas reinhardtii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 30-140fold | Chlamydomonas reinhardtii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | mating gamete | Chlamydomonas reinhardtii | - |
| vegetative cell | proenzyme | Chlamydomonas reinhardtii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-neoendorphin + H2O | - |
Chlamydomonas reinhardtii | ? | - |
? | |
| dynorphin(1-13) + H2O | - |
Chlamydomonas reinhardtii | ? | - |
? | |
| Mastoparan + H2O | - |
Chlamydomonas reinhardtii | ? | - |
? | |
| neurotensin + H2O | - |
Chlamydomonas reinhardtii | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cell wall lytic enzyme | - |
Chlamydomonas reinhardtii |
| Gamete autolysin | - |
Chlamydomonas reinhardtii |
| Gamete lytic enzyme | - |
Chlamydomonas reinhardtii |
| GLE | - |
Chlamydomonas reinhardtii |
| mmp1 | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Chlamydomonas reinhardtii |
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