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Literature summary for 3.4.24.38 extracted from

  • Matsuda, Y.; Saito, T.; Yamaguchi, T.; Kawase, H.
    Cell wall lytic enzyme released by mating gametes of Chlamydomonas reinhardtii is a metalloprotease and digests the sodium perchlorate-insoluble component of cell wall (1985), J. Biol. Chem., 260, 6373-6377.
    View publication on PubMed
Search for more literature for 3.4.24.38

Inhibitors

Inhibitors Comment Organism Structure
aspartate
-
 Chlamydomonas reinhardtii
CDTA
-
 Chlamydomonas reinhardtii
Co2+ above 1-2 mM, native or apoenzyme Chlamydomonas reinhardtii
Cu(CH3COO)2
-
 Chlamydomonas reinhardtii
cysteine
-
 Chlamydomonas reinhardtii
EDTA inactivation by dialysis, 1-2 mM Zn2+, Mn2+ or Co2+ restores activity of metall-free apoenzyme to 0.5%, 10% and 50% of native enzyme, respectively, no restoring by Mg2+, Ca2+, Cu2+ or Fe3+; most effectively reversed by Co2+ Chlamydomonas reinhardtii
EGTA about 25% as effective as EDTA or CDTA Chlamydomonas reinhardtii
glutamate
-
 Chlamydomonas reinhardtii
histidine
-
 Chlamydomonas reinhardtii
Mn2+ above 1-2 mM, native or apoenzyme Chlamydomonas reinhardtii
additional information chymostatin, antipain, leupeptin; pepstatin A; various amino acids, protease inhibitor E-64 Chlamydomonas reinhardtii
phosphoramidon
-
 Chlamydomonas reinhardtii
Zn2+ above 1-2 mM, native or apoenzyme Chlamydomonas reinhardtii

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not contain cobalt or manganese Chlamydomonas reinhardtii
Zinc zinc enzyme, 2.5 gatom zinc/62000 MW enzyme subunit, atomic absorption spectroscopy Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, Chlamydomonas reinhardtii, SDS-PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Cell wall protein + H2O Chlamydomonas reinhardtii released during agglutination of gametes of opposite mating type ?
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
 green alga, wild-type strain 137c
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information isolated from gametes Chlamydomonas reinhardtii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O poor substrate Chlamydomonas reinhardtii fragments of azocasein
-
 ?
casein + H2O alpha- and beta-casein, poor substrate Chlamydomonas reinhardtii polypeptides derived from casein
-
 ?
Cell wall protein + H2O released during agglutination of gametes of opposite mating type Chlamydomonas reinhardtii ?
-
 ?
Chlamydomonas cell wall polypeptide + H2O 2 polypeptides (peptide 2, MW 294000, and 5, MW 215000) of 16 polypeptides, i.e. the NaClO4-insoluble fraction, disintegrates cell wall structure by acting on only a few salt-insoluble components Chlamydomonas reinhardtii ?
-
 ?
additional information bovine serum albumin Chlamydomonas reinhardtii ?
-
 ?
additional information no substrates are cytochrome c, lysozyme, myoglobin, trypsin inhibitor, ovalbumin Chlamydomonas reinhardtii ?
-
 ?
RNAse A + H2O
-
 Chlamydomonas reinhardtii Lower MW polypetides
-
 ?
Zoosporangia cell walls + H2O glutaraldehyde-fixed Chlamydomonas reinhardtii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, Chlamydomonas reinhardtii, SDS-PAGE Chlamydomonas reinhardtii