Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.37 extracted from

  • dEnfert, C.; Ibrahim-Granet, O.
    Saccharolysin (2004), Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press, 1, 360-361.
No PubMed abstract available
Search for more literature for 3.4.24.37

Application

Application Comment Organism
degradation most likely major intracellular oligopeptidase responsible for the degradation of peptides resulting from nonvacuolar proteolysis Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information prd1-6 mutants with thermolabile saccharolysin activity, prd1::URA3 mutants devoid of the cytoplasmic and mitochondrial activities Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxymercuribenzoate 100% inhibition with 0.1 mM Saccharomyces cerevisiae
Cpp-Ala-Ala-Phe-p-aminobenzoic acid strongly inhibits Saccharomyces cerevisiae
EDTA inhibits 50% at 1 mM and 90% at 10 mM, can be efficiently reactivated by the addition of Zn2+, Co2+ and Mn2+ Saccharomyces cerevisiae
HgCl2 100% inhibition with 0.01 mM Saccharomyces cerevisiae
additional information not inhibited by DTT, N-ethylmaleimide, iodoacetic acid or iodoacetamide Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 Pz-Pro-Leu-Gly-Pro-D-Arg
-
 Saccharomyces cerevisiae
0.029
-
 Dnp-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Saccharomyces cerevisiae
0.035
-
 MeOSuc-Ala-Ala-Pro-Met-NHPhNO2
-
 Saccharomyces cerevisiae
0.06
-
 Bz-Pro-Phe-Arg-NHPhNO2
-
 Saccharomyces cerevisiae
0.085
-
 Ac-Ala-Ala-Pro-Phe-NHPhNO2
-
 Saccharomyces cerevisiae
0.19
-
 Ac-Ala-Ala-Pro-Met-NHPhNO2
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Saccharomyces cerevisiae 5829
-
mitochondrion intermembrane space Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81800
-
-
 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
250fold by chromatographies Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-Ala-Ala-Pro-Met-NHPhNO2 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
Ac-Ala-Ala-Pro-Phe-NHPhNO2 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
bradykinin + H2O cleaved at the Phe-Ser bond Saccharomyces cerevisiae ?
-
 ?
Bz-Pro-Phe-Arg-NHPhNO2 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
Dnp-Pro-Leu-Gly-Pro-Trp-D-Lys + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
Mcc-Pro-Leu-Gly-Pro-D-Lys(Dnp) + H2O
-
 Saccharomyces cerevisiae Mcc-Pro-Leu + Gly-Pro-D-Lys(Dnp)
-
 ?
MeOSuc-Ala-Ala-Pro-Met-NHPhNO2 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
additional information no cleavage of peptides with a free N-terminus, azocasein and (3H)methylcasein Saccharomyces cerevisiae ?
-
 ?
Pz-Pro-Leu-Gly-Pro-D-Arg + H2O
-
 Saccharomyces cerevisiae Pz-Pro-Leu + Gly-Pro-D-Arg
-
 ?
Pz-Pro-Leu-Gly-Pro-D-Arg + H2O
-
 Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
EC 3.4.22.22 formerly Saccharomyces cerevisiae
Prd1
-
 Saccharomyces cerevisiae
yscD
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.5
-
 with MeOSuc-Ala-Ala-Pro-Met-NHPhNO2 Saccharomyces cerevisiae
5.9
-
 with Ac-Ala-Ala-Pro-Met-NHPhNO2 Saccharomyces cerevisiae
7.9
-
 with Bz-Pro-Phe-Arg-NHPhNO2 Saccharomyces cerevisiae