Literature summary for 3.4.24.36 extracted from

Schlagenhauf, E.; Etges, R.; Metcalf, P.
Crystallization and preliminary X-ray diffraction studies of leishmanolysin, the major surface metalloproteinase from Leishmania major (1995), Proteins Struct. Funct. Genet., 22, 58-66.

Search for more literature for 3.4.24.36

Crystallization (Commentary)

Crystallization (Comment)	Organism
Leishmania major, crystallographic parameters	Leishmania major

General Stability

General Stability	Organism
Resistant to proteolytic degradation	Leishmania major

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	glycosylphosphatidylinositol-anchored	Leishmania major	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	microheterogeneity in MW and charge	Leishmania major
52000	-	x * 52000, Leishmania major, deglycosylated enzyme, x * 53200, Leishmania major, deglycosylated enzyme, SDS-PAGE	Leishmania major
52570	-	Leishmania major, calculated from nucleotide sequence plus carbohydrate core of GPI anchor	Leishmania major
53200	-	x * 52000, Leishmania major, deglycosylated enzyme, x * 53200, Leishmania major, deglycosylated enzyme, SDS-PAGE	Leishmania major

Organism

Organism	UniProt	Comment	Textmining
Leishmania major	-	non-infective strains LRC-119	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	1-8% carbohydrate	Leishmania major

Source Tissue

Source Tissue	Comment	Organism	Textmining
promastigote	lipophosphoglycan-deficient stationary phase promastigote	Leishmania major	-

Storage Stability

Storage Stability	Organism
4°C, 20 mg enzyme/ml, in 10 mM Tris-HCl buffer, pH 8, 0.02% w/v NaN3, prolonged periods of time	Leishmania major

Subunits

Subunits	Comment	Organism
?	x * 52000, Leishmania major, deglycosylated enzyme, x * 53200, Leishmania major, deglycosylated enzyme, SDS-PAGE	Leishmania major

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	isoelectric pattern, microheterogeneity in MW and charge	Leishmania major

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Release 2023.1 (January 2023)