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Literature summary for 3.4.24.35 extracted from

  • Patchigolla, R.; Knudson, W.; Schmid, T.
    Matrix metalloproteinase-9 in a unique proteoglycan form in avian embryonic growth plate cartilage (2012), Arch. Biochem. Biophys., 520, 42-50.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
aminophenylmercuric acetate
-
Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline strong inhibition at 1 mM Gallus gallus
EDTA complete inhibition at 10 mM Gallus gallus
macroglobulin
-
Gallus gallus
marimastat
-
Gallus gallus
additional information the 230 kDa enzyme does not remain intact in 4 M urea, 0.5 M NaCl, 4 M CsCl or 4 M GdHCl. Glycosaminoglycan chains may interfere with inhibitors Gallus gallus
tissue inhibitor of metalloproteinases 1 TIMP-1, complete inhibition at 0.5 mg Gallus gallus
tissue inhibitor of metalloproteinases 2 TIMP-2, complete inhibition at 0.5 mg Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 80000, deglycosylated enzyme, SDS-PAGE, x * 230000, glycosylated enzyme, SDS-PAGE Gallus gallus
230000
-
x * 80000, deglycosylated enzyme, SDS-PAGE, x * 230000, glycosylated enzyme, SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Gelatin + H2O Gallus gallus
-
?
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information the 230 kDa enzyme does not remain intact in 4 M urea, 0.5 M NaCl, 4 M CsCl or 4 M GdHCl Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme contains covalently bound glycosaminoglycan i.e. chondroitin sulfate chains, deglycosylation by protease-free chondroitinase ABC Gallus gallus
additional information digestion of the intact enzyme with chondroitinase decreases the size of the molecule to 80 kDa Gallus gallus

Purification (Commentary)

Purification (Comment) Organism
native enzyme from tibia by gelatin-agarose affinity chromatography, anion exchange chromatography, and dialysis Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
bone
-
Gallus gallus
-
cartilage embryonic Gallus gallus
-
embryo
-
Gallus gallus
-
tibia
-
Gallus gallus
-
tibial cartilage
-
Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxy-coumarin-4-yl)acetyl-PLGL-beta-(2,4-dinitrophenylamino)-AAR-NH2 + H2O
-
Gallus gallus (7-methoxy-coumarin-4-yl)acetyl-PL + GL-beta-(2,4-dinitrophenylamino)-AAR-NH2
-
?
Gelatin + H2O
-
Gallus gallus ?
-
?

Subunits

Subunits Comment Organism
? x * 80000, deglycosylated enzyme, SDS-PAGE, x * 230000, glycosylated enzyme, SDS-PAGE Gallus gallus

Synonyms

Synonyms Comment Organism
gelatinase
-
Gallus gallus
gelatinolytic enzyme
-
Gallus gallus
MMP-9
-
Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Gallus gallus

Expression

Organism Comment Expression
Gallus gallus the enzyme expression is induced in chick embryonic tibias cultured with lipopolysaccharide up

General Information

General Information Comment Organism
additional information digestion of the intact enzyme with chondroitinase decreases the size of the molecule to 80 kDa Gallus gallus