Activating Compound | Comment | Organism | Structure |
---|---|---|---|
aminophenylmercuric acetate | - |
Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | strong inhibition at 1 mM | Gallus gallus | |
EDTA | complete inhibition at 10 mM | Gallus gallus | |
macroglobulin | - |
Gallus gallus | |
marimastat | - |
Gallus gallus | |
additional information | the 230 kDa enzyme does not remain intact in 4 M urea, 0.5 M NaCl, 4 M CsCl or 4 M GdHCl. Glycosaminoglycan chains may interfere with inhibitors | Gallus gallus | |
tissue inhibitor of metalloproteinases 1 | TIMP-1, complete inhibition at 0.5 mg | Gallus gallus | |
tissue inhibitor of metalloproteinases 2 | TIMP-2, complete inhibition at 0.5 mg | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, deglycosylated enzyme, SDS-PAGE, x * 230000, glycosylated enzyme, SDS-PAGE | Gallus gallus |
230000 | - |
x * 80000, deglycosylated enzyme, SDS-PAGE, x * 230000, glycosylated enzyme, SDS-PAGE | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | Gallus gallus | - |
? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | the 230 kDa enzyme does not remain intact in 4 M urea, 0.5 M NaCl, 4 M CsCl or 4 M GdHCl | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme contains covalently bound glycosaminoglycan i.e. chondroitin sulfate chains, deglycosylation by protease-free chondroitinase ABC | Gallus gallus |
additional information | digestion of the intact enzyme with chondroitinase decreases the size of the molecule to 80 kDa | Gallus gallus |
Purification (Comment) | Organism |
---|---|
native enzyme from tibia by gelatin-agarose affinity chromatography, anion exchange chromatography, and dialysis | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone | - |
Gallus gallus | - |
cartilage | embryonic | Gallus gallus | - |
embryo | - |
Gallus gallus | - |
tibia | - |
Gallus gallus | - |
tibial cartilage | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxy-coumarin-4-yl)acetyl-PLGL-beta-(2,4-dinitrophenylamino)-AAR-NH2 + H2O | - |
Gallus gallus | (7-methoxy-coumarin-4-yl)acetyl-PL + GL-beta-(2,4-dinitrophenylamino)-AAR-NH2 | - |
? | |
Gelatin + H2O | - |
Gallus gallus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, deglycosylated enzyme, SDS-PAGE, x * 230000, glycosylated enzyme, SDS-PAGE | Gallus gallus |
Synonyms | Comment | Organism |
---|---|---|
gelatinase | - |
Gallus gallus |
gelatinolytic enzyme | - |
Gallus gallus |
MMP-9 | - |
Gallus gallus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | assay at | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Gallus gallus |
Organism | Comment | Expression |
---|---|---|
Gallus gallus | the enzyme expression is induced in chick embryonic tibias cultured with lipopolysaccharide | up |
General Information | Comment | Organism |
---|---|---|
additional information | digestion of the intact enzyme with chondroitinase decreases the size of the molecule to 80 kDa | Gallus gallus |