Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes in Hep-G2 cells using recombinant adenoviral vectors adenovirus-2 or adenovirus-5, secretion of the recombinant enzymes, overview | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
E402H/H411E | site-directed mutagenesis, proteolytic inactive MMP-9 mutant, application of the MMP-9 mutant decreases portal and periportal accumulation of collagen and inhibits fibrogenesis induced by CCl4 in mice | Mus musculus |
E402Q | site-directed mutagenesis, proteolytic inactive MMP-9 mutant, application of the MMP-9 mutant decreases portal and periportal accumulation of collagen and inhibits fibrogenesis induced by CCl4 in mice, the MMP-9 mutant suppresses transdifferentiation of hepatic stellate cells to the myofibroblast-like phenotype in vitro and in vivo. Adenoviral application of the mutant leads to increased apoptosis of activated hepatic stellate cells | Mus musculus |
H401A | site-directed mutagenesis, proteolytic inactive MMP-9 mutant, application of the MMP-9 mutant decreases portal and periportal accumulation of collagen and inhibits fibrogenesis induced by CCl4 in mice, the MMP-9 mutant suppresses transdifferentiation of hepatic stellate cells to the myofibroblast-like phenotype in vitro and in vivo. Adenoviral application of the mutant leads to increased apoptosis of activated hepatic stellate cells | Mus musculus |
additional information | MMP-9 mutants reduce type I collagen protein, TIMP-1, and MMP-2 expression in vivo, overview | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tissue inhibitor of metalloproteinases-1 | i.e. TIMP-1, plays a crucial role in the pathogenesis of hepatic fibrosis and thus may represent an important therapeutic target in the design of anti-fibrotic strategiesfor chronic liver disease, overview, molecular modeling of the three-dimensional structure of the MMP-9/TIMP-1 complex, overview | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
Balb/c mice | - |
Mus musculus BALB/c | - |
Balb/c mice | - |
Purification (Comment) | Organism |
---|---|
recombinant secreted mutant enzymes from Hep-G2 cells by gelatin affinity chromatography to homogeneity | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Subunits | Comment | Organism |
---|---|---|
More | molecular modeling of the three-dimensional structure of the MMP-9/TIMP-1 complex, overview | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
matrix metalloproteinase-9 | - |
Mus musculus |
MMP-9 | - |
Mus musculus |