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Literature summary for 3.4.24.34 extracted from

  • Fasciglione, G.F.; Marini, S.; D'Alessio, S.; Politi, V.; Coletta, M.
    pH- and temperature-dependence of functional modulation in metalloproteinases. A comparison between neutrophil collagenase and gelatinases A and B (2000), Biophys. J., 79, 2138-2149.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0194
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-L-diaminopropionyl-Ala-Arg-NH2 pH 5.0-8.0, 37°C, 1-protonated substrate Homo sapiens
0.028
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-L-diaminopropionyl-Ala-Arg-NH2 pH 5.0-8.0, 37°C, 2-protonated substrate Homo sapiens
0.0465
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-L-diaminopropionyl-Ala-Arg-NH2 pH 5.0-8.0, 37°C, 3-protonated and 4-protonated substrate Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ present in the active site, involved in teh catalytic mechanism Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-diaminopropionyl-Ala-Arg-NH2 + H2O
-
 Homo sapiens ?
-
 ?
(7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-L-diaminopropionyl-Ala-Arg-NH2 + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
MMP-8
-
 Homo sapiens
neutrophil interstitial collagenase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
145
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-diaminopropionyl-Ala-Arg-NH2 pH 5.0-8.0, 37°C, 4-protonated substrate Homo sapiens
164
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-diaminopropionyl-Ala-Arg-NH2 pH 5.0-8.0, 37°C, unprotonated substrate Homo sapiens
342
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-diaminopropionyl-Ala-Arg-NH2 pH 5.0-8.0, 37°C, 2-protonated substrate Homo sapiens
522
-
 (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-diaminopropionyl-Ala-Arg-NH2 pH 5.0-8.0, 37°C, 3-protonated substrate Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 8
-
 Homo sapiens