Literature summary for 3.4.24.33 extracted from

Sonomura, K.; Kuyama, H.; Matsuo, E.; Tsunasawa, S.; Futaki, S.; Nishimura, O.
Selective isolation of N-blocked peptide by combining AspN digestion, transamination, and tosylhydrazine glass treatment (2011), Anal. Biochem., 410, 214-223.

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Application

Application	Comment	Organism
molecular biology	many eukaryotic proteins are blocked at the alpha-amino group of their N-terminal with various modifications, thereby making it difficult to determine their N-terminal sequence by protein sequencer, development of a method for selectively isolating the blocked N-terminal peptide from the peptide mixture generated by endoproteinase AspN digestion of N-blocked protein by removal of all peptides other than the N-terminal one (non-N-terminal peptides) through their carbonyl group introduced by a chemical transamination reaction	Pseudomonas fragi

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fragi	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	recombinant enzyme	Pseudomonas fragi	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	high substrate specificity of AspN, that ensures that all of the non-N-terminal peptides having aspartic acid or glutamic acid at their N-termini can be converted. An artificially targeted N-blocked protein is digested with AspN, method overview. The proposed method is applicable to proteins, whether N blocked or N free	Pseudomonas fragi	?	-	?

Synonyms

Synonyms	Comment	Organism
AspN	-	Pseudomonas fragi
endoproteinase AspN	-	Pseudomonas fragi

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Release 2023.1 (January 2023)