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Literature summary for 3.4.24.32 extracted from
- Bacteriolytic activity and specificity of Achromobacter beta-lytic protease (1998), J. Biochem., 124, 332-339.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| blp gene | Achromobacter lyticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 90% inhibition | Achromobacter lyticus |  |
| 2-mercaptoethanol | 66% inhibition | Achromobacter lyticus | |
| additional information | only 2% inhibition with EDTA, no inhibition with diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride, iodoacetate, phosphoramidon, N-chloroacetyl-N-hydroxy-leucyl-Ala-Gly-NH2, chymostatin, elastinal, leupeptin, and pepstatin | Achromobacter lyticus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | one zinc atom detected in the molecule by ion-spray mass spectrometry | Achromobacter lyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19280 | - |
acetonitrile-inactivated enzyme, ion spray mass spectrometry | Achromobacter lyticus |
| 19280 | - |
calculated from the amino acid sequence deduced from the nucleotide sequence of the cloned blp gene | Achromobacter lyticus |
| 19350 | - |
active enzyme, ion spray mass spectrometry | Achromobacter lyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Achromobacter lyticus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DYMGWMDF-NH2 + H2O | - |
Achromobacter lyticus | DYMG + WMDF-NH2 | - |
? | |
| GNLWATGHFM-NH2 + H2O | - |
Achromobacter lyticus | GNLWATG + HFM-NH2 | - |
? | |
| KFIGLM-NH2 + H2O | - |
Achromobacter lyticus | KFIG + Leu-Met-NH2 | - |
? | |
| L-pyroglutamyl-HWSYGLRPG-NH2 + H2O | - |
Achromobacter lyticus | L-pyroglutamyl-HWSYG + LRPG-NH2 | - |
? | |
| L-pyroglutamyl-LM-NH2 + H2O | - |
Achromobacter lyticus | ? | - |
? | |
| L-pyroglutamyl-QRLGNQWAVGHLM-NH2 + H2O | - |
Achromobacter lyticus | L-pyroglutamyl-QRLG + NQWAVGHLM-NH2 | - |
? | |
| L-pyroglutamyl-VPQWAVGHFM-NH2 + H2O | - |
Achromobacter lyticus | L-pyroglutamyl-VPQWAVG + HFM-NH2 | - |
? | |
| RPPGFSPFR + H2O | - |
Achromobacter lyticus | RPPG + FSPFR | - |
? | |
| WAGGDASGE + H2O | - |
Achromobacter lyticus | WAGG + DASGE | - |
? | |
| WAGGNASGE + H2O | - |
Achromobacter lyticus | WAGG + NASGE | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Achromopeptidase component | - |
Achromobacter lyticus |
| beta-lytic metalloproteinase | - |
Achromobacter lyticus |
| beta-Lytic protease | - |
Achromobacter lyticus |
| blp | - |
Achromobacter lyticus |
| EC 3.4.24.4 | formerly included in | Achromobacter lyticus |
| EC 3.4.99.13 | formerly | Achromobacter lyticus |
| Myxobacter AL-1 proteinase I | formerly EC 3.4.99.29 | Achromobacter lyticus |
| Myxobacter beta-lytic proteinase | - |
Achromobacter lyticus |
| Myxobacter495 beta-lytic proteinase | - |
Achromobacter lyticus |
| Myxobacterium sorangium beta-lytic proteinase | - |
Achromobacter lyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
peptide substrates | Achromobacter lyticus |
| 10 | - |
bacterial substrates | Achromobacter lyticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10.5 | exhibits higher bacteriolytic activity at pH 10.0 than at pH 7.0-9.0 | Achromobacter lyticus |
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