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Literature summary for 3.4.24.32 extracted from

  • Jackson, R.L.; Matsueda, G.R.
    Myxobacter AL-1 protease (1970), Methods Enzymol., 19, 591-599.
No PubMed abstract available
Search for more literature for 3.4.24.32

Inhibitors

Inhibitors Comment Organism Structure
additional information citrate; dinitrofluorobenzene; EDTA; iodoacetic acid; not: PCMB unidentified myxobacterium

Organism

Organism UniProt Comment Textmining
unidentified myxobacterium
-
-
-
unidentified myxobacterium AL-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
strain AL-1 unidentified myxobacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 unidentified myxobacterium

Storage Stability

Storage Stability Organism
4°C, 0.02 M Tris-HCl, pH 9.0, stable for at least 8 h unidentified myxobacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Azocoll + H2O
-
 unidentified myxobacterium Hydrolyzed azocoll
-
 ?
Azocoll + H2O
-
 unidentified myxobacterium AL-1 Hydrolyzed azocoll
-
 ?
Bacterial cell walls + H2O Arthrobacter crystallopoietes cells walls unidentified myxobacterium Hydrolyzed bacterial cell walls
-
 ?
Bacterial cell walls + H2O Arthrobacter crystallopoietes cells walls unidentified myxobacterium AL-1 Hydrolyzed bacterial cell walls
-
 ?
Insulin B-chain + H2O hydrolysis of Ala-Leu bond unidentified myxobacterium Hydrolyzed insulin B-chain
-
 ?
Insulin B-chain + H2O hydrolysis of Ala-Leu bond unidentified myxobacterium AL-1 Hydrolyzed insulin B-chain
-
 ?
additional information affinity towards peptide bonds formed by at least one hydrophobic amino acid in a structure at least as large as a tetrapeptide unidentified myxobacterium ?
-
 ?
additional information affinity towards peptide bonds formed by at least one hydrophobic amino acid in a structure at least as large as a tetrapeptide unidentified myxobacterium AL-1 ?
-
 ?
pentaglycine + H2O
-
 unidentified myxobacterium ?
-
 ?
pentaglycine + H2O
-
 unidentified myxobacterium AL-1 ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 0.02 M Tris-HCl, pH 9.0, 13% loss of activity after 8 h unidentified myxobacterium
40 50 0.02 M Tris-HCl, pH 9.0, 70% loss of activity after 8 h unidentified myxobacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 cell wall lysis, azocoll unidentified myxobacterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 1 h, 95% loss of activity unidentified myxobacterium
6.5 9.5 45°C, 1 h, maximal stability unidentified myxobacterium